阿糖胞苷或柔红霉素与全反式维A酸的不同联用方式致体外HL-60凋亡差异

张春; 陆晓彤; 陈敏玲; 张顺国; 王燕琼; 赵薇; 张健

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

重要提示

《药学实践与服务》杂志不接收生物战剂、毒剂相关内容的稿件。对于涉军信息类论文如需投稿，请务必投稿前电话或邮箱咨询。联系电话：021-81871323，邮箱：yxsjzzs@163.com。敬请谅解，谢谢配合！

《药学实践与服务》编辑部

免责声明

《药学实践与服务》杂志目前不收取审稿费、版面费、加急费等费用，如收到邮件声称是编辑部X编辑，要求加作者微信的，请谨防财产损失！编辑部用于作者校稿时微信绑定的邮件是通过yxsjzzs@163.com发送的，标题是《药学实践与服务》XML数字出版服务微信绑定，请区分开！

阿糖胞苷或柔红霉素与全反式维A酸的不同联用方式致体外HL-60凋亡差异

张春, 陆晓彤, 陈敏玲, 张顺国, 王燕琼, 赵薇, 张健

文章导航 > 药学实践与服务 > 2010 > 28(4): 262-264,273

张春, 陆晓彤, 陈敏玲, 张顺国, 王燕琼, 赵薇, 张健. 阿糖胞苷或柔红霉素与全反式维A酸的不同联用方式致体外HL-60凋亡差异[J]. 药学实践与服务, 2010, 28(4): 262-264,273.

引用本文:

ZHANG Chun, LU Xiao-tong, CHEN Min-ling, ZHANG Shun-guo, WANG Yan-qiong, ZHAO Wei, ZHANG Jian. Different administrations of combination cytarabine or daunorubicin with all-trans retinoic acid lead to variance of HL-60''s apoptosis and respectively mechanism in vitro[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(4): 262-264,273.

Citation:

ZHANG Chun, LU Xiao-tong, CHEN Min-ling, ZHANG Shun-guo, WANG Yan-qiong, ZHAO Wei, ZHANG Jian. Different administrations of combination cytarabine or daunorubicin with all-trans retinoic acid lead to variance of HL-60''s apoptosis and respectively mechanism in vitro[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(4): 262-264,273.

阿糖胞苷或柔红霉素与全反式维A酸的不同联用方式致体外HL-60凋亡差异

1.
上海交通大学医学院附属新华医院,上海200092
2.
上海交通大学医学院附属上海儿童医学中心,上海200127

Different administrations of combination cytarabine or daunorubicin with all-trans retinoic acid lead to variance of HL-60''s apoptosis and respectively mechanism in vitro

1.
Xinhua Hospital offiliated to Shanghai Jiaotong University Medical School,Shanghai 200092,Chain
2.
Department of Pharmacy,Shanghai Children's Medical Center affiliated to Shanghai Jiaotong University Medical School,Shanghai 200127,China

摘要: 目的: 研究阿糖胞苷(cytarabine,Ara-C)或柔红霉素(daunorubicine,DNR)与全反式维A酸(all-trans retinoicacid,ATRA)以不同方式联用,体外作用于白血病细胞株HL-60,导致细胞凋亡差异及可能机制。 方法: 体外培养HL-60细胞株,细胞悬液以浓度2.0×10⁵/ml接种后分7组,分别为A:对照组;B:Ara-C与ATRA同时联用48 h组,Ara-C+ATRA48 h;C:ATRA与Ara-C先后联用48 h组,ATRA 24 h→Ara-C 24 h;D:Ara-C与ATRA先后联用48 h组,Ara-C 24 h→ATRA 24 h;E:DNR与ATRA同时联用48 h组,DNR+ATRA48 h;F:ATRA与DNR先后联用48 h组,ATRA24 h→DNR24 h;G:DNR与AT-RA先后联用48 h组,DNR 24 h→ATRA24 h。Ara-C、DNR、ATRA给药浓度均为1.0×10^-7mol/L。应用流式细胞仪、Westernblotting观察药物不同方式联用致HL-60细胞凋亡的差异及相关机制变化。 结果: 与A组相比,D组HL-60细胞凋亡比例轻度增加(50±14.7)%,抗凋亡蛋白Bcl-2消失;E组和G组HL-60凋亡细胞明显增多,其中E组能最大限度减少HL-60存活数量,使HL-60细胞死亡数目增加,达(4.00±0.56)%;E、F、G组抗凋亡蛋白Bcl-2均消失。 结论: 同时联用DNR与ATRA能显著减少HL-60细胞存活率,两药的三种联用方式均可使抗凋亡蛋白Bcl-2消失。但是,首先应用小剂量Ara-C,随后应用AT-RA的联用方式可明显增多细胞凋亡数目,抑制HL-60细胞的抗凋亡蛋白Bcl-2不能表达,有效控制细胞凋亡方向,是最有潜力的药物联用方式。
- 阿糖胞苷 /
- 柔红霉素 /
- 全反式维A酸 /
- HL-60 /
- 联用 /
- 凋亡 /
- 抗凋亡蛋白
Abstract: Objective To investigate variance of apoptosis and correspondingly mechanism in HL-60 cell line in vitro by different combinations adminstration of three drug including cytarabine,daunorubicin and all-trans retinoic acid.Methonds HL-60 cell line was cultured in vitro,cell suspension was divided into different 7 groups which's density was 2.0×10⁵/ml : Group A: control;Group B:Ara-C and ATRA were simultaneous applied in cells for 48 h(Ara-C+ATRA 48 h);Group C: ATRA and Ara-C subsequently administrated respectively for 24 h that is ATRA was applied first for 24 h,then followed by Ara-C for 24 h.Group D: Ara-C and ATRA subsequently administrated respectively for 24 h that is Ara-C was applied first for 24 h,then followed by ATRA for 24 h;Group E: DNR and ATRA simultaneously administrated for 48 h;Group F: DNR and ATRA subsequently administrated respectively for 24 h that is DNR was applied first for 24 h,then followed by ATRA for 24 h.Group G: ATRA and DNR subsequently administrated respectively for 24 h that is ATRA was applied first for 24 h,then followed by DNR for 24 h.All drug concentrations of Ara-C,DNR and ATRA were 1.0×10^-7 mol/L.The ratio of apoptosis cells was evaluated by flow cytometry.Anti-apoptosis protein Bcl-2 was checked by Western Blotting. Results The ratio of cell apoptosis in D group increase slightly,the rate of increase in apoptosis was(50±14.7) %,its protein Bcl-2 disappeared.Compared with control group,both two groups including E and G groups caused the apoptosis ratio significant higher.The alive cells' ratio in E group was reduced to the highest degree and the amount of dead cells increased to a degree of(4.00±0.56) %.Anti-apoptosis protein Bcl-2 in E,F,G groups all disappeared. Conclusion Combination DNR and ATRA simultaneously can effectively decrease HL-60 survival.Three ways of combination DNR with ATRA can signifi- cantly cause protein Bcl-2 disappear.But low dose of Ara-C was administrated first then followed by ATRA can not only increase slightly the ratio of apoptosis of HL-60 cell,but also inhibited anti-apoptosis protein Bcl-2 to express and effectively controled the way of HL-60 cell's death to apoptosis,and we concluded that this combination is the most potentiall way.
- cytarabine /
- daunorubicin /
- all-trans retinoic acid /
- HL-60 /
- combination /
- apoptosis /
- anti-apoptosis protein

[1]	张霖峰, 孙宇恒, 王冬尧, 李丹, 曹岩, 吕狄亚. 茵陈蒿汤体内成分分析及其抗肝癌作用机制的网络药理学研究 . 药学实践与服务, 2025, 43(): 1-9. doi: 10.12206/j.issn.2097-2024.202501017
[2]	邱彦, 吴浩, 董雅芬, 陈业, 王建, 金辉. 新型二氢卟吩化合物的光动力性能及其抗肺癌作用研究 . 药学实践与服务, 2025, 43(): 1-7. doi: 10.12206/j.issn.2097-2024.202304011
[3]	吴若南, 叶爽, 李墨晨轩, 缪震元, 罗川. 冬凌草甲素磺酰脲衍生物的设计与抗炎活性研究 . 药学实践与服务, 2025, 43(7): 335-338, 352. doi: 10.12206/j.issn.2097-2024.202401048
[4]	陶宫佳, 陈林林, 宋泽成, 刘梦肖, 王彦. 苦参碱及衍生物的抗炎作用及其机制研究进展 . 药学实践与服务, 2025, 43(4): 163-168, 194. doi: 10.12206/j.issn.2097-2024.202406035
[5]	宋泽成, 马闪闪, 胡巧灵, 仲华, 王彦. 小檗碱与氟康唑合用抗氟康唑耐受白念珠菌的研究 . 药学实践与服务, 2025, 43(2): 87-91. doi: 10.12206/j.issn.2097-2024.202409047
[6]	韦薇, 张明霞, 周艳萍, 颜岚, 田鹏, 冯霞. 基于失效模式与效应分析优化静脉用药调配中心全流程质量控制体系 . 药学实践与服务, 2025, 43(): 1-7. doi: 10.12206/j.issn.2097-2024.202504131
[7]	史生辉, 刘潇, 陈栋, 暨迪军, 马倩, 卢永昌. 二维液相色谱法分离藏药木藤蓼的化学成分 . 药学实践与服务, 2025, 43(9): 443-448. doi: 10.12206/j.issn.2097-2024.202412065
[8]	魏莱, 董国强. SARS-CoV-2主蛋白酶抑制剂和降解剂研究进展 . 药学实践与服务, 2025, 43(6): 259-269, 297. doi: 10.12206/j.issn.2097-2024.202503064
[9]	詹济华, 颜滢. 低蛋白血症对头孢哌酮舒巴坦血药浓度的影响 . 药学实践与服务, 2025, 43(7): 344-347. doi: 10.12206/j.issn.2097-2024.202309040
[10]	魏莱, 董国强, 盛春泉. 靶向SARS-CoV-2主蛋白酶的PROTAC设计、合成与蛋白降解活性研究 . 药学实践与服务, 2025, 43(5): 235-241, 258. doi: 10.12206/j.issn.2097-2024.202503063
[11]	关梦瑶, 夏天爽, 何旭辉, 史策, 蒋益萍, 辛海量. 黑蒜多糖抗便秘作用研究 . 药学实践与服务, 2025, 43(4): 190-194. doi: 10.12206/j.issn.2097-2024.202403059
[12]	冯婷婷, 张景翔, 王彦, 许维恒, 张俊平. ANXA3基因及蛋白的研究进展 . 药学实践与服务, 2025, 43(2): 47-50, 74. doi: 10.12206/j.issn.2097-2024.202309023
[13]	曹奇, 张嘉宝, 王培. 基于无监督自动降维分析与手动圈门联用的骨骼肌髓系细胞多色流式分析方法 . 药学实践与服务, 2025, 43(7): 325-328, 366. doi: 10.12206/j.issn.2097-2024.202404077
[14]	竺东杰, 贺新征, 邹杰, 余史丹, 李红霞. 雷公藤甲素对大鼠脑缺血再灌注损伤的影响及机制研究 . 药学实践与服务, 2025, 43(7): 339-343, 361. doi: 10.12206/j.issn.2097-2024.202311021
[15]	张俊丽, 李媛媛, 尹静, 杨鸿源, 白耀武. 咪达唑仑调节PINK1/PARKIN信号通路对缺血性脑卒中大鼠神经元损伤的影响 . 药学实践与服务, 2025, 43(6): 288-292, 312. doi: 10.12206/j.issn.2097-2024.202405024
[16]	白学鑫, 陈玉平, 盛春泉, 武善超. 具核梭杆菌小分子抑制剂的筛选及其抗结直肠癌活性研究 . 药学实践与服务, 2024, 42(12): 503-507. doi: 10.12206/j.issn.2097-2024.202405009
[17]	张林晨, 张小琴, 张俊平. 山楂酸药理作用的研究进展 . 药学实践与服务, 2024, 42(5): 185-189. doi: 10.12206/j.issn.2097-2024.202307052
[18]	刘汝雄, 杨万镇, 涂杰, 盛春泉. 铁死亡调控蛋白GPX4的小分子抑制剂研究进展 . 药学实践与服务, 2024, 42(9): 375-378. doi: 10.12206/j.issn.2097-2024.202312075
[19]	修建平, 杨朝爱, 刘禧澳, 潘乾禹, 韦广旭, 王卫星. 全反式维甲酸对肝星状细胞活化及氧化应激的作用和机制探索 . 药学实践与服务, 2024, 42(7): 291-296. doi: 10.12206/j.issn.2097-2024.202312054
[20]	冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军. 雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055

点击查看大图

计量

文章访问数: 3089
HTML全文浏览量: 323
PDF下载量: 583
被引次数: 0

阿糖胞苷或柔红霉素与全反式维A酸的不同联用方式致体外HL-60凋亡差异

1. 上海交通大学医学院附属新华医院,上海200092

2. 上海交通大学医学院附属上海儿童医学中心,上海200127

收稿日期: 2009-12-11
修回日期: 2010-01-11

关键词:

阿糖胞苷 /

柔红霉素 /

全反式维A酸 /

HL-60 /

联用 /

凋亡 /

抗凋亡蛋白

摘要: 目的: 研究阿糖胞苷(cytarabine,Ara-C)或柔红霉素(daunorubicine,DNR)与全反式维A酸(all-trans retinoicacid,ATRA)以不同方式联用,体外作用于白血病细胞株HL-60,导致细胞凋亡差异及可能机制。 方法: 体外培养HL-60细胞株,细胞悬液以浓度2.0×10⁵/ml接种后分7组,分别为A:对照组;B:Ara-C与ATRA同时联用48 h组,Ara-C+ATRA48 h;C:ATRA与Ara-C先后联用48 h组,ATRA 24 h→Ara-C 24 h;D:Ara-C与ATRA先后联用48 h组,Ara-C 24 h→ATRA 24 h;E:DNR与ATRA同时联用48 h组,DNR+ATRA48 h;F:ATRA与DNR先后联用48 h组,ATRA24 h→DNR24 h;G:DNR与AT-RA先后联用48 h组,DNR 24 h→ATRA24 h。Ara-C、DNR、ATRA给药浓度均为1.0×10^-7mol/L。应用流式细胞仪、Westernblotting观察药物不同方式联用致HL-60细胞凋亡的差异及相关机制变化。 结果: 与A组相比,D组HL-60细胞凋亡比例轻度增加(50±14.7)%,抗凋亡蛋白Bcl-2消失;E组和G组HL-60凋亡细胞明显增多,其中E组能最大限度减少HL-60存活数量,使HL-60细胞死亡数目增加,达(4.00±0.56)%;E、F、G组抗凋亡蛋白Bcl-2均消失。 结论: 同时联用DNR与ATRA能显著减少HL-60细胞存活率,两药的三种联用方式均可使抗凋亡蛋白Bcl-2消失。但是,首先应用小剂量Ara-C,随后应用AT-RA的联用方式可明显增多细胞凋亡数目,抑制HL-60细胞的抗凋亡蛋白Bcl-2不能表达,有效控制细胞凋亡方向,是最有潜力的药物联用方式。

English Abstract

Different administrations of combination cytarabine or daunorubicin with all-trans retinoic acid lead to variance of HL-60''s apoptosis and respectively mechanism in vitro

1. Xinhua Hospital offiliated to Shanghai Jiaotong University Medical School,Shanghai 200092,Chain

2. Department of Pharmacy,Shanghai Children's Medical Center affiliated to Shanghai Jiaotong University Medical School,Shanghai 200127,China

Received Date: 2009-12-11
Rev Recd Date: 2010-01-11

Keywords:

Abstract: Objective To investigate variance of apoptosis and correspondingly mechanism in HL-60 cell line in vitro by different combinations adminstration of three drug including cytarabine,daunorubicin and all-trans retinoic acid.Methonds HL-60 cell line was cultured in vitro,cell suspension was divided into different 7 groups which's density was 2.0×10⁵/ml : Group A: control;Group B:Ara-C and ATRA were simultaneous applied in cells for 48 h(Ara-C+ATRA 48 h);Group C: ATRA and Ara-C subsequently administrated respectively for 24 h that is ATRA was applied first for 24 h,then followed by Ara-C for 24 h.Group D: Ara-C and ATRA subsequently administrated respectively for 24 h that is Ara-C was applied first for 24 h,then followed by ATRA for 24 h;Group E: DNR and ATRA simultaneously administrated for 48 h;Group F: DNR and ATRA subsequently administrated respectively for 24 h that is DNR was applied first for 24 h,then followed by ATRA for 24 h.Group G: ATRA and DNR subsequently administrated respectively for 24 h that is ATRA was applied first for 24 h,then followed by DNR for 24 h.All drug concentrations of Ara-C,DNR and ATRA were 1.0×10^-7 mol/L.The ratio of apoptosis cells was evaluated by flow cytometry.Anti-apoptosis protein Bcl-2 was checked by Western Blotting. Results The ratio of cell apoptosis in D group increase slightly,the rate of increase in apoptosis was(50±14.7) %,its protein Bcl-2 disappeared.Compared with control group,both two groups including E and G groups caused the apoptosis ratio significant higher.The alive cells' ratio in E group was reduced to the highest degree and the amount of dead cells increased to a degree of(4.00±0.56) %.Anti-apoptosis protein Bcl-2 in E,F,G groups all disappeared. Conclusion Combination DNR and ATRA simultaneously can effectively decrease HL-60 survival.Three ways of combination DNR with ATRA can signifi- cantly cause protein Bcl-2 disappear.But low dose of Ara-C was administrated first then followed by ATRA can not only increase slightly the ratio of apoptosis of HL-60 cell,but also inhibited anti-apoptosis protein Bcl-2 to express and effectively controled the way of HL-60 cell's death to apoptosis,and we concluded that this combination is the most potentiall way.

引用本文:

Citation:

ZHANG Chun, LU Xiao-tong, CHEN Min-ling, ZHANG Shun-guo, WANG Yan-qiong, ZHAO Wei, ZHANG Jian. Different administrations of combination cytarabine or daunorubicin with all-trans retinoic acid lead to variance of HL-60''s apoptosis and respectively mechanism in vitro[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(4): 262-264,273.

全文HTML

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

网站备案号沪ICP备05003363号-1

本系统由北京仁和汇智信息技术有限公司开发

留言板

阿糖胞苷或柔红霉素与全反式维A酸的不同联用方式致体外HL-60凋亡差异

Different administrations of combination cytarabine or daunorubicin with all-trans retinoic acid lead to variance of HL-60''s apoptosis and respectively mechanism in vitro

计量

出版历程

阿糖胞苷或柔红霉素与全反式维A酸的不同联用方式致体外HL-60凋亡差异

1. 上海交通大学医学院附属新华医院,上海200092 2. 上海交通大学医学院附属上海儿童医学中心,上海200127

English Abstract

Different administrations of combination cytarabine or daunorubicin with all-trans retinoic acid lead to variance of HL-60''s apoptosis and respectively mechanism in vitro

1. Xinhua Hospital offiliated to Shanghai Jiaotong University Medical School,Shanghai 200092,Chain 2. Department of Pharmacy,Shanghai Children's Medical Center affiliated to Shanghai Jiaotong University Medical School,Shanghai 200127,China

全文HTML

目录

1. 上海交通大学医学院附属新华医院,上海200092

2. 上海交通大学医学院附属上海儿童医学中心,上海200127

1. Xinhua Hospital offiliated to Shanghai Jiaotong University Medical School,Shanghai 200092,Chain

2. Department of Pharmacy,Shanghai Children's Medical Center affiliated to Shanghai Jiaotong University Medical School,Shanghai 200127,China