欧芹素乙对人脐静脉内皮细胞的保护作用及对血管内皮生长因子表达的影响
Inhibitory effects of vascular endothelial growth factor expression on human umbilical vein endothelial cells by imperatorin
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摘要: 目的:研究欧芹素乙对内皮细胞的保护作用;溶血磷脂酰胆碱(LPC)对人脐静脉内皮细胞株HUVEC细胞中血管内皮生长因子(VEGF)表达的影响以及欧芹素乙的影响。方法:应用四唑盐(MTT)法检测溶血磷脂酰胆碱对HUVEC细胞的毒性作用及欧芹素乙的保护作用;应用基础酶联免疫吸附试验(ELISA)检测各组条件培养基中VEGF蛋白含量;采用RT-PCR法及Realtime PCR方法检测溶血磷脂酰胆碱对VEGF mRNA的表达及欧芹素乙的影响。结果:MTT检测结果显示,溶血磷脂酰胆碱对HUVEC细胞具有较强的生长抑制作用,而欧芹素乙对溶血磷脂酰胆碱所致的细胞增殖抑制具有较好的保护作用。ELISA结果显示,HUVEC细胞暴露于溶血磷脂酰胆碱后,VEGF蛋白含量明显升高;加入欧芹素乙后剂量依赖性地降低VEGF蛋白的表达。RT-PCR结果显示,溶血磷脂酰胆碱可以增加3种VEGF异构体的转录水平,其中VEGF165的表达显著增加,欧芹素乙可剂量依赖性地抑制溶血磷脂酰胆碱引起的VEGF mRNA的高表达。结论:欧芹素乙对溶血磷脂酰胆碱引起的细胞损伤有明显的保护作用;欧芹素乙可抑制溶血磷脂酰胆碱所诱导的HUVEC细胞中VEGF蛋白及VEGF mRNA的高表达,对内皮细胞起到保护作用。Abstract: Objective : To study the protective effects of imperatorin(IMP) against human umbilical vein endothelial cells(HUVEC) damage and expression of vascular endothelial growth factor(VEGF) induced by lysophosphatidvlcholine(LPC). Methods : Cell iniury were determined by MTT assay.Vascular endothelial growth factor protein production from HUVEC was determined by enzyme-linked immunosorbent assay(ELISA).VEGF mRNA expression was examined by RT-PCR and realtime PCR. Results :Imperatorin prevented LPC-induced injury in cultured HUVEC in a concentration-dependent manner.LPC induced expression of VEGF protein and VEGF mRNA on HUVEC.Imperatorin could inhibit the expression of VEGF protein an d VEGF mRNA in co-cultured with LPC. Conclusions :LPC could induce cell damage and a strong expression of VEGF on HUVEC.Imperatorin could protect HUVEC against the LPC-induced damage and downregulate VEGF protein and VEGF mRNA expression in cultured HUVEC.
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Key words:
- lysophosphatidylcholines /
- endothelial growth factors /
- imperatorin
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