聚酰胺柱层析法分离回心草总黄酮的研究
Studies of isolation for total flavonoids in Rhodobryum giganteum by polyamide column adsorption chromatogtaphy
-
摘要: 目的:确定聚酰胺柱层析法分离回心草总黄酮的条件。方法:对聚酰胺用量、乙醇洗脱浓度、洗脱速度等方面进行考察。结果:干法上样,聚酰胺用量以药材:聚酰胺=1:1为佳,以50%的乙醇为洗脱溶剂,2倍柱体积/h为最佳的洗脱速率。结论:聚酰胺可较好地用于回心草总黄酮的分离富集。Abstract: Objective : To select the optimal condition of polyamide column adsorption chromatogtaphy for separating the total flavonoids in Rhodobryum giganteum(Schwaegr.) Par. Methods :The amount of polyamide,the eluting reagent and the eluting rate were studied. Results :It was suitable when the herb amount:polyamide amount was 11,and 50% ethnoal was the sutiable eluting reagent.The flow rate of eluent was 2 times volume of column/h. Conclusion :polyamide is suitable for the separation and collection of the total flavonoids from Rhodobryum giganteum(Schwaegr.) Par.
-
Key words:
- Rhodobryum giganteum(Schwaegr.) Par. /
- polyamide /
- total flavonoids
-
点击查看大图
计量
- 文章访问数: 2530
- HTML全文浏览量: 175
- PDF下载量: 726
- 被引次数: 0
下载:
