不同产地大黄药材的近红外漫反射光谱法鉴别
Identification of Rheum officinale by near infrared diffuse reflectance spectroscopy
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摘要: 目的 建立用近红外漫反射光谱鉴别不同产地大黄药材的新方法.方法 采集不同产地的大黄药材及其伪品的近红外漫反射光谱,分别用OPUS软件自带的聚类分析组件与第二军医大学药学院研发的近红外光谱-褶合变换-信息可视化-相似系数分析软件对其进行鉴别.结果 聚类分析的结果不甚理想,褶合变换分析得到正品与伪品大黄的相似系数<0.68,而正品大黄药材之间的相似系数均>0.81,同产地的药材之间的相似系数均>0.92.结论 近红外光谱法简便、快速,结合褶合变换分析得到的相似系数能够准确鉴别正品、伪品以及不同产地的大黄药材,所得分析结果与经典的形态分类学鉴别方法一致.Abstract: Objective To establish a near infrared diffuse reflectance spectroscopic (NIRDRS) method for the identification of Rheum officinale Baill from different production area.Method The near infrared spectra of Rheum officinale Baill from different origin and Rh:franzenbachii munt were treated respectively by cluster analysis provided by OPUS software and convolution transform-visualization-similarity (CVS) analysis developed by SMMU.Result Cluster analysis resulted in some confusion of the medicinal materials with each other, while the CVS analysis provided a better resolution.The similarity coefficients of the authentic and pseudo herbs were less than 0.68.The coefficients within the authentic herbs were greater than 0.81, while those within the same production area were greater than 0.92.Conclusion The simple and fast NIRDRS combined with CVS analysis provided a new approach to identify Rheum officinale Baill from different origin and Rh:franzenbachii munt which coincided quite well with the traditional morphological classification of the traditional Chinese medicines.
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Key words:
- Rheum officinale Baill /
- near infrared spectroscopy /
- identification
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