High purity preparation and identification of three phthalide compounds from Apium graveolens
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摘要: 目的 分离和鉴定芹菜籽中苯酞类化合物。 方法 采用硅胶柱色谱、制备薄层色谱方法分离纯化苯酞类化学成分，用波谱分析技术鉴定化合物结构。 结果 从芹菜籽中分离制备得到3种主要的苯酞类化合物，分别鉴定为芹菜甲素、芹菜乙素和新蛇床内酯。 结论 采用该法制备的样品纯度大于98%，可作为对照品使用。Abstract: Objective Isolation, purification and structure identification of phthalides from Apium graveolens L. Methods Silica gel column and preparative TLC chromatography were used to isolate and purify the phthalide compounds. The structures of those compounds were determined through spectral analysis. Results Three pure compounds were identified as 3-n-butylphthalide, sedanenolide and sedanolide. Conclusion Three pure phthalide compounds can be used as analytical reference standard for quantitative analysis of A. graveolens.
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Key words:
- Apium graveolens /
- 3-n-butylphthalide /
- sedanenolide /
- sedanolide /
- structure identification
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[1] 吾不力卡斯木·吾不力艾山. 芹菜的心血管药理作用及临床应用分析[J]. 中国民族民间医药, 2011, 20(19):35. [2] 王文宝, 马华夏. 芹菜籽化学成分及药理作用的研究进展[J]. 吉林医药学院学报, 2012, 33(1):49-51. [3] 张会群. 丁苯酞的药理作用与药动学研究及临床评价[J]. 医学信息, 2014(39):266. [4] 廖艳彪, 彭立辉, 卢明. 丁基苯酞对血管性痴呆的认知功能的影响[J]. 医学综述, 2014, 20(18):3343-3345. [5] 端木寅, 王源, 梁爽, 等. 丁苯酞脑缺血治疗作用的相关药理学研究进展[J]. 中药药理与临床, 2012(3):126-130. [6] Yang H, Hu GY, Chen J,et al. Synthesis, resolution, and antiplatelet activity of 3-substituted 1(3H)-isobenzofuranone[J]. Bioorg Med Chem Lett, 2007, 17(18):5210-5213. [7] Fazal SS, Ansari MM, Singla RK, et al. Isolation of 3-n-Butyl Phthalide & Sedanenolide from Apium graveolens Linn[J]. Indo Glob J Pharm Sci, 2012, 2(3):258-261. [8] Wang J, Yang JB, Wang AG, et al. Studies on the chemical constituents of Lgusticum sinense[J]. J Chin Med Mater, 2011, 34(3):378-380. -
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