Determination of baicalin in Kangdeling capsule by HPLC
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摘要: 目的 建立康得灵胶囊中黄芩苷的HPLC测定方法。 方法 色谱柱为Agilent Tc-C18色谱柱（4.6 mm×250 mm，5 μm），柱温为30 ℃；流动相为乙腈-0.5‰磷酸溶液（26：74），流速为1.0 ml/min，检测波长265 nm。 结果 黄芩苷保留时间约为16 min。以峰面积（Y）对进样浓度（X， μg/ml）线性回归，得回归方程：Y=22 114.67 X－112 836.7，r=0.998 8，线性范围5.410~108.2 μg/ml。平均加样回收率为98.78%，RSD为0.74%。 结论 本方法操作简便，测定结果准确可靠，可用于康得灵胶囊中黄芩苷的含量测定。Abstract: Objective To establish an HPLC quantitative method for the content determination of baicalin in Kangdeling capsule. Methods The chromatography column was Agilent Tc-C18-WR (4.6 mm×250 mm,5 μm),and the column temperature was 30 ℃.The mobile phase consisted of acetonitrile and 0.5‰ phosphoric acid (26:74).The flow rate was 1.0 ml/min, and the detection wavelength was 265 nm. Results The retention time of baicalin was about 16 min. The calibration equation was Y=22 114.67 X-112 836.7(r=0998 8)with good linearity in the range of 5.410-108.2 μg/ml for baicalin. The average recovery was 98.78% while RSD were 0.74%. Conclusion This method is simple, time-saving and accurate which could be used to routine analysis of baicalin in Kangdeling capsule.
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[1] 陈玉敏,曹 红,路军章,等.HPLC法测定复方竹叶石膏颗粒中野黄芩苷的含量[J].解放军药学学报,2013,29(4): 365-366. [2] 李永钦,周井炎,徐辉碧.黄芩苷的抗氧化作用[J].华中理工大学学报,1999,27(4):110-112. [3] 王 瑞,李玉娟,毕开顺,等.HP-HPLC法测定解毒直透汤中连翘苷、绿原酸及黄芩苷的含量[J].中药材,2004,24(4):287-288. [4] 李守拙,李沈明,范书泽.黄芩茎叶中野黄芩苷含量测定[J].中国医药杂志,2008,6(5):40-41. [5] 舒德忠,郑小敏,胜明智.高效液相色谱法测定消炎止咳糖浆中黄芩苷的含量[J].中国药师,2008,11(8):947-948. [6] 薛黎明,秦雪梅,张丽增.不同产地黄芩药材的黄芩苷含量测定及指纹图谱研究[J].中成药,2008,30(1):10-13. -
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