血余炭止血包止血效果的实验研究
Experimental study on hemostasis effect of carbonized hair
-
摘要: 目的 观察血余炭止血包的止血作用。 方法 以兔子为实验对象,采用试管法测定体外凝血时间;用巴马小型猪建立其肝脏、脾脏、股动、静脉创伤出血模型,观察血余炭止血包的止血效果。 结果 体外凝血实验,与云南白药组、空白对照组和阴性对照淀粉组比较,血余炭组体外凝血时间明显缩短(P<0.05,P<0.01)。在巴马小型猪创伤止血实验中,与阴性对照淀粉组比较,血余炭止血包能明显缩短巴马小型猪肝脏、脾脏创伤出血时间(P<0.01),并明显减少出血量(P<0.01)。与云南白药组和阴性对照淀粉组比较,血余炭组对巴马小型猪股动、静脉创伤出血有显著性止血作用(P<0.05,P<0.01)。组织学观察,股动脉创面组织周围未见明显病理改变。 结论 血余炭止血包有较好的凝血效果,在巴马小型猪肝脏、脾脏创伤出血模型中能明显缩短出血时间,减少出血量,止血实验效果显著;能有效控制模型中股动、静脉致命性出血,降低死亡率。Abstract: Objective To observe the hemostasis effect of carbonized hair. Methods The coagulation time was measured in vitro with blood collected from rabbits in the presence of blank, starch, carbonized hair or Yunnan White Drug-Powder respectively. Bama miniature pigs traumatic model was established and animal haemostatic experiment was performed.Hemostatic effect was observed in liver, spleen and femoral arteriovenous fistula hemorrhagic model of Bama miniature pigs. Results The coagulation time of rabbit blood was markedly shorter by addition of carbonized hair compared to Yunnan White Drug-Powder, starch and blank (P<0.05,P<0.01).Compare with the starch group, carbonized hair could significantly shorten the bleeding time and decrease the volume of bleeding (P<0.01), in the liver and spleen of hemorrhagic model of Bama miniature pigs. Compared with the Yunnan White Drug-Powder and the starch, carbonized hair could more effectively stop the femoral arteriovenous fistula bleeding in the model of Bama miniature pigs (P<0.05,P<0.01). Histologic study revealed there was no significant pathological change around the femoral artery. Conclution Carbonized hair could effectively inhibit blood coagulation in vitro, decrease bleeding volume and bleeding time in the liver and spleen of hemorrhagic model of Bama miniature pigs. Carbonized hair dressing could also effectively control hemorrhage in femoral arteriovenous fistula model with lethal vascular injury.
-
Key words:
- carbonized hair /
- hemostasis /
- bleeding /
- artery /
- wound
-
[1] 汉瑞娟,王志红,桂 莉.战伤救治新进展[J].中国误诊学杂志,2007,7(5):940. [2] 王 蕾,李武平,孙惠英,等.战伤急救止血技术新进展[J].解放军护理杂志,2007,24(12):45. [3] 王 建,葛宝丰,刘兴炎,等.战伤止血方法及材料研究进展[J].人民军医,2008,51(1):5. [4] 李纳英.血余炭质量现状及煅制浅析[J]. 现代中药研究与实践,2008,22(3):52. [5] 景冬樱,张文仁,卞 俊,等.复凝粉止血作用实验研究[J].解放军药学学报,2004,20(6):445. [6] 曹永孝,马 朝,朱少非,等.急救止血敷片的止血作用[J].西安交通大学学报(医学版),2010,31(2):169. [7] 毕宏达,李学拥,王 玉,等.沸石对猪血管损伤止血的作用及对组织影响的初步研究[J].第四军医大学学报,2006,27(9):851. [8] Rohtwell WS,Fudge J M,Chen WK,et al.Addition of a propylgallate-based procoagulant to a fibrin bandage improves hemostatic performance in a swine arterial bleeding model[J].Thromb Res,2002,108(5-6):335. [9] Rothwell SW,Fudge JM,Reid TJ,et al. Epsilon-amino caproic acid additive decreases fibrin bandage performance in a swine arterial bleeding model[J]. Thromb Res,2002,108(5-6):341. [10] 颜克松,战大伟,王 巍,等.一次性使用速效止血包对小型猪止血疗效试验观察[J].中国畜牧兽医,2010,37(6):239. [11] 毕宏达,李学拥,王 玉,等.沸石对猪致命性血管损伤模型的止血作用[J].西北国防医学杂志,2006,27(3):164. [12] 董小胜,黄洁靖,张 林.中药血余炭的研究进展[J].中医药导报,2009,15(12):85. [13] Sondeen JL,Pusateri AE,Coppes VG,et al.Comparison of 10 different hemostatic dressings in an aortic injury[J].J Trauma,2003,54:280.
计量
- 文章访问数: 2897
- HTML全文浏览量: 240
- PDF下载量: 183
- 被引次数: 0