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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2017  >  35(1): 43-47,53

XIE Xinfang, WEN Yan, GAO Shouhong, YOU Chunhua, CHEN Wansheng, XIONG Xiaojuan. An in vitro study of hepatotoxicity induced by valproic acid and its metabolites[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(1): 43-47,53. doi: 10.3969/j.issn.1006-0111.2017.01.011
Citation: XIE Xinfang, WEN Yan, GAO Shouhong, YOU Chunhua, CHEN Wansheng, XIONG Xiaojuan. An in vitro study of hepatotoxicity induced by valproic acid and its metabolites[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(1): 43-47,53. doi: 10.3969/j.issn.1006-0111.2017.01.011
shu

An in vitro study of hepatotoxicity induced by valproic acid and its metabolites

doi: 10.3969/j.issn.1006-0111.2017.01.011
  • 1.

    Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;College of Chemical and Biological Engineering, Yichun University, Yichun 336000, China

  • 2.

    Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China

  • 3.

    College of Chemical and Biological Engineering, Yichun University, Yichun 336000, China

  • Received Date: 2016-05-31
  • Rev Recd Date: 2016-09-29
  • Objective To confirm the hepatotoxicity of valproic acid (VPA) and its metabolites(2-propyl-4-pentenoic acid, 3-hydroxy valproic acid, 5-hydroxy valproic acid) on human liver cells. Methods Cells were divided into control group and VPA-treated group. The control group was conventionally cultured while the VPA-treated group was treated with valproic acid and its metabolites. The rate of cell proliferation was assayed by CCK 8 protocol. The mRNA levels of CYP1A1, CYP1A2, PCNA, Bax and Bcl-2 were measured by real time PCR. The correlated protein levels were measured by Western Blotting. The activity of LDH, AST and ALT were also detected. Results Compared to the control group, with the increases of concentrations and reaction time of VPA and its metabolites, the proliferation rate of L02-cell was reduced, the mRNA and protein levels of CYP1A1, CYP1A2, and Bax was increased, the mRNA and protein level of PCNA and Bcl-2 was decreased, AST, ALT, and LDH were also elevated in the treated group. Conclusion Valproic acid and its metabolites were positively related to hepatotoxicity.
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    [16] 陈海鹰,曹雨诞,颜晓静,等. 醋制降低京大戟对人正常肝细胞L02的毒性及机制研究[J].中国中药杂志,2013,38(6):866-870.
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An in vitro study of hepatotoxicity induced by valproic acid and its metabolites

doi: 10.3969/j.issn.1006-0111.2017.01.011
  • 1. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;College of Chemical and Biological Engineering, Yichun University, Yichun 336000, China
  • 2. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
  • 3. College of Chemical and Biological Engineering, Yichun University, Yichun 336000, China
  • Received Date: 2016-05-31
  • Rev Recd Date: 2016-09-29

Abstract: Objective To confirm the hepatotoxicity of valproic acid (VPA) and its metabolites(2-propyl-4-pentenoic acid, 3-hydroxy valproic acid, 5-hydroxy valproic acid) on human liver cells. Methods Cells were divided into control group and VPA-treated group. The control group was conventionally cultured while the VPA-treated group was treated with valproic acid and its metabolites. The rate of cell proliferation was assayed by CCK 8 protocol. The mRNA levels of CYP1A1, CYP1A2, PCNA, Bax and Bcl-2 were measured by real time PCR. The correlated protein levels were measured by Western Blotting. The activity of LDH, AST and ALT were also detected. Results Compared to the control group, with the increases of concentrations and reaction time of VPA and its metabolites, the proliferation rate of L02-cell was reduced, the mRNA and protein levels of CYP1A1, CYP1A2, and Bax was increased, the mRNA and protein level of PCNA and Bcl-2 was decreased, AST, ALT, and LDH were also elevated in the treated group. Conclusion Valproic acid and its metabolites were positively related to hepatotoxicity.

XIE Xinfang, WEN Yan, GAO Shouhong, YOU Chunhua, CHEN Wansheng, XIONG Xiaojuan. An in vitro study of hepatotoxicity induced by valproic acid and its metabolites[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(1): 43-47,53. doi: 10.3969/j.issn.1006-0111.2017.01.011
Citation: XIE Xinfang, WEN Yan, GAO Shouhong, YOU Chunhua, CHEN Wansheng, XIONG Xiaojuan. An in vitro study of hepatotoxicity induced by valproic acid and its metabolites[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(1): 43-47,53. doi: 10.3969/j.issn.1006-0111.2017.01.011
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