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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2016  >  34(3): 255-257

CHEN Bi, HUANG Xiaoliang, LI Hui. Determination of valproate in serum by precolumn derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 255-257. doi: 10.3969/j.issn.1006-0111.2016.03.016
Citation: CHEN Bi, HUANG Xiaoliang, LI Hui. Determination of valproate in serum by precolumn derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 255-257. doi: 10.3969/j.issn.1006-0111.2016.03.016
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Determination of valproate in serum by precolumn derivatization HPLC

doi: 10.3969/j.issn.1006-0111.2016.03.016

  • Department of Pharmacy, the First People's Hospital of Chenzhou, Chenzhou 423000, China

  • Received Date: 2014-10-31
  • Rev Recd Date: 2015-04-09
  • Objective To establish a determination method for valprate in serum. Methods Serum sample was acidified by sulfuric acid and extracted with ethyl ether, cyclohexanecarboxylic acid was selected as an internal standard, α-bromoacetophenone as derivative reagent. Determination was performed with HPLC with methanol:water (70:30) as the mobile phase.The nalytical column was Eclipse Plus C18 (150 mm×4.6 mm, 5 μm), detected at 248 nm, the flow rate was 1.0 ml/min. Results The linear rang of valproate was 8.65~173 μg/ml. The mean relative recovery was bigger than 99.27%. Both the relative standard deviation (RSD) of inter-day and intra-day was less than 5%. Conclusions The method is rapid, accurate, sensitive and suitable for clinical therapeutic drug monitoring.
  • [1] 孙利伟,闫红月. 荧光偏振免疫法测定丙戊酸钠血清药物浓度[J].中国实用医药,2010,5(29):127-128.
    [2] 汤方萍,王伟娇,李艳飞. 气相色谱法同时测定丙戊酸钠和托吡酯血药浓度[J].中国医院药学杂志,2010,30(8):674-675.
    [3] 杨春兰,方会慧,夏泉,等. 柱前衍生化-HPLC法测定患者体内丙戊酸钠的血药浓度[J].安徽医药,2011,15(11):1352-1354.
    [4] 阳波,李湘斌. 柱前衍生高效液相色谱法测定人血清中丙戊酸钠浓度[J].中南药学,2006,4(5):360-362.
    [5] 李顺炜,毛名扬,李国忠. 改进的柱前衍生-高效液相色谱法测定人血中丙戊酸钠[J].中国医药药学杂志,2005,25(8):713-714.
    [6] 郭丽萍,李霞. 高效液相色谱法测定人血清中丙戊酸钠浓度[J].中国药师,2007,10(7):723-725.
    [7] 孙增先,张骞峰,周金玉,等.柱前衍生-高效液相色谱法测定丙戊酸钠血药浓度[J].中国临床药学杂志,2004,13(2):93-95.
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Determination of valproate in serum by precolumn derivatization HPLC

doi: 10.3969/j.issn.1006-0111.2016.03.016
  • Department of Pharmacy, the First People's Hospital of Chenzhou, Chenzhou 423000, China
  • Received Date: 2014-10-31
  • Rev Recd Date: 2015-04-09

Abstract: Objective To establish a determination method for valprate in serum. Methods Serum sample was acidified by sulfuric acid and extracted with ethyl ether, cyclohexanecarboxylic acid was selected as an internal standard, α-bromoacetophenone as derivative reagent. Determination was performed with HPLC with methanol:water (70:30) as the mobile phase.The nalytical column was Eclipse Plus C18 (150 mm×4.6 mm, 5 μm), detected at 248 nm, the flow rate was 1.0 ml/min. Results The linear rang of valproate was 8.65~173 μg/ml. The mean relative recovery was bigger than 99.27%. Both the relative standard deviation (RSD) of inter-day and intra-day was less than 5%. Conclusions The method is rapid, accurate, sensitive and suitable for clinical therapeutic drug monitoring.

CHEN Bi, HUANG Xiaoliang, LI Hui. Determination of valproate in serum by precolumn derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 255-257. doi: 10.3969/j.issn.1006-0111.2016.03.016
Citation: CHEN Bi, HUANG Xiaoliang, LI Hui. Determination of valproate in serum by precolumn derivatization HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 255-257. doi: 10.3969/j.issn.1006-0111.2016.03.016
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