Identification and determination of scutellarin in compound granule tetrandra

ZHANG Shuyu; XU Jianjiang; YU Yanli; BI Yunsheng; ZHAO Wenjing; LIU Qian; LIU Tongqi

doi:10.3969/j.issn.1006-0111.2016.03.013

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Objective To develop the methods for identification and determination of scutellarin in compound Granule Tetrandra. Methods A TLC method was used to identify quality for scutellarin, and a HPLC method was used to determine the content of scutellarin. Results The spot was clear in TLC identification without interference of negative control. The sample size of scutellarin had a good linear relationship with peak area r=0.9996(n=5)when ranged from 22.4~156.8 μg/ml, with the average recovery rate 97.79%(RSD=0.32%, n=6). Conclusion The method is simple, with good specificity and reproducibility, which can be used as the quality control for this preparation.

Identification and determination of scutellarin in compound granule tetrandra

Department of Pharmacy, Jinan Military General Hospital, Jinan 250031, China

Received Date: 2014-12-01

Rev Recd Date: 2015-03-23

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Abstract: Objective To develop the methods for identification and determination of scutellarin in compound Granule Tetrandra. Methods A TLC method was used to identify quality for scutellarin, and a HPLC method was used to determine the content of scutellarin. Results The spot was clear in TLC identification without interference of negative control. The sample size of scutellarin had a good linear relationship with peak area r=0.9996(n=5)when ranged from 22.4~156.8 μg/ml, with the average recovery rate 97.79%(RSD=0.32%, n=6). Conclusion The method is simple, with good specificity and reproducibility, which can be used as the quality control for this preparation.

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Identification and determination of scutellarin in compound granule tetrandra

doi: 10.3969/j.issn.1006-0111.2016.03.013

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