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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2016  >  34(3): 223-226,277

XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008
Citation: XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008
shu

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

doi: 10.3969/j.issn.1006-0111.2016.03.008
  • 1.

    AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China;School of Life Sciences, Fudan University, Shanghai 200438, China

  • 2.

    AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China

  • 3.

    School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China

  • Received Date: 2015-06-26
  • Rev Recd Date: 2015-10-21
  • Objective To study the cell proliferative effects of fungal immunomodulatory proteins from Ganoderma spp. on 26 gastric cancer cell lines in vitro. Method 26 human gastric cancer cell lines were treated with FIPs by MTS assay. The average optical density(OD) in 490 nm and inhibition rate(GI50)was counted by Universal Microplate Spectrophotometer. Results Three FIPs showed similar profiling in 26 human gastric cancer cell lines after 72 h treatment in cell proliferation assay, which except for NUGC-4 and OCUM-1 did not showed obvious anti-proliferative effect, the other 24 human gastric cell lines showed some anti-proliferative effects, especially for 7 cell lines(NUGC-3, GTL-16, HGC-27, IM95m, SNU-638, SNU-216 and SNU-5) showing strong potency, with their GI50 less than 50 μg/ml. Conclusion FIPs showed strong anti-proliferative effects in some human gastric cancer cell lines in vitro, which had potential to be further developed as anti-gastric cancer drugs.
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    [2] Li F, Wen H, Zhang Y, et al. Purification and characterization of a novel immunomodulatory protein from the medicinal mushroom Trametes versicolor [J]. Sci China Life Sci, 2011, 54(4):379-385.
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    [4] Li QZ, Huang L, Wang XF, et al. Fungal immunomodulatory protein from Flammulina velutipes induces cytokine gene expression in mouse spleen cells[J]. Curr Top Nutraceut R, 2011, 9(3):111-118.
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    [6] 王晓林, 梁重阳, 李泓睿, 等. 灵芝免疫调节蛋白(rLZ-8)诱导K562细胞发生细胞核介导的细胞凋亡的研究[J]. 中国免疫学杂志, 2010, 26(7):616-618.
    [7] Liao CH, Hsiao YM, Lin CH, et al. Induction of premature senescence in human lung cancer by fungal immunomodulatory protein from Ganoderma tsugae[J]. Food Chem Toxicol, 2008, 46(5):1851-1859.
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    [9] 刘建文.抗肿瘤药理实验方法[M].北京:化学工业出版社,2010:29.
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    [11] Tanaka M, Obata T, Sasaki T.Evaluation of antitumour effects of docetaxel (Taxotere) on human gastric cancers in vitro and in vivo [J]. Eur J Cancer, 1996, 32A (2):226-230.
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    [15] Ishiyama M, Tominaga H, Shiga M, et al. Novel cell proliferation and cytotoxicity assays using a tetrazolium salt that produces a comparison of multiple cytotoxicity assays 61 water-soluble formazan dye[J]. In Vitro Toxicol, 1995, 8(2):187-190.
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Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

doi: 10.3969/j.issn.1006-0111.2016.03.008
  • 1. AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China;School of Life Sciences, Fudan University, Shanghai 200438, China
  • 2. AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China
  • 3. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China
  • Received Date: 2015-06-26
  • Rev Recd Date: 2015-10-21

Abstract: Objective To study the cell proliferative effects of fungal immunomodulatory proteins from Ganoderma spp. on 26 gastric cancer cell lines in vitro. Method 26 human gastric cancer cell lines were treated with FIPs by MTS assay. The average optical density(OD) in 490 nm and inhibition rate(GI50)was counted by Universal Microplate Spectrophotometer. Results Three FIPs showed similar profiling in 26 human gastric cancer cell lines after 72 h treatment in cell proliferation assay, which except for NUGC-4 and OCUM-1 did not showed obvious anti-proliferative effect, the other 24 human gastric cell lines showed some anti-proliferative effects, especially for 7 cell lines(NUGC-3, GTL-16, HGC-27, IM95m, SNU-638, SNU-216 and SNU-5) showing strong potency, with their GI50 less than 50 μg/ml. Conclusion FIPs showed strong anti-proliferative effects in some human gastric cancer cell lines in vitro, which had potential to be further developed as anti-gastric cancer drugs.

XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008
Citation: XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008
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