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Volume 34 Issue 3
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2016  >  34(3): 223-226,277

SHEN Liru, LI Ruihong, WANG Tailiang. Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
Citation: XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008
shu

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

doi: 10.3969/j.issn.1006-0111.2016.03.008
  • 1.

    AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China;School of Life Sciences, Fudan University, Shanghai 200438, China

  • 2.

    AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China

  • 3.

    School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China

  • Received Date: 2015-06-26
  • Rev Recd Date: 2015-10-21
  • Objective To study the cell proliferative effects of fungal immunomodulatory proteins from Ganoderma spp. on 26 gastric cancer cell lines in vitro. Method 26 human gastric cancer cell lines were treated with FIPs by MTS assay. The average optical density(OD) in 490 nm and inhibition rate(GI50)was counted by Universal Microplate Spectrophotometer. Results Three FIPs showed similar profiling in 26 human gastric cancer cell lines after 72 h treatment in cell proliferation assay, which except for NUGC-4 and OCUM-1 did not showed obvious anti-proliferative effect, the other 24 human gastric cell lines showed some anti-proliferative effects, especially for 7 cell lines(NUGC-3, GTL-16, HGC-27, IM95m, SNU-638, SNU-216 and SNU-5) showing strong potency, with their GI50 less than 50 μg/ml. Conclusion FIPs showed strong anti-proliferative effects in some human gastric cancer cell lines in vitro, which had potential to be further developed as anti-gastric cancer drugs.
  • [1] 苏恺琪, 王雪飞, 周选围. 黑灵芝免疫调节蛋白基因的克隆和生物信息学分析[J]. 上海交通大学学报(农业科学版), 2012, 30(1):65-71.
    [2] Li F, Wen H, Zhang Y, et al. Purification and characterization of a novel immunomodulatory protein from the medicinal mushroom Trametes versicolor [J]. Sci China Life Sci, 2011, 54(4):379-385.
    [3] Wang XF, Li QZ, Bao TW, et al. In vitro rapid evolution of fungal immunomodulatory proteins by DNA family shuffling[J]. Appl Microbiol Biotechnol, 2013, 97(6):2455-2465.
    [4] Li QZ, Huang L, Wang XF, et al. Fungal immunomodulatory protein from Flammulina velutipes induces cytokine gene expression in mouse spleen cells[J]. Curr Top Nutraceut R, 2011, 9(3):111-118.
    [5] Li QZ, Wang XF, Zhou XW. Recent status and prospects of the fungal immunomodulatory protein family[J]. Crit Rev Biotechnol, 2011, 31(4):365-375.
    [6] 王晓林, 梁重阳, 李泓睿, 等. 灵芝免疫调节蛋白(rLZ-8)诱导K562细胞发生细胞核介导的细胞凋亡的研究[J]. 中国免疫学杂志, 2010, 26(7):616-618.
    [7] Liao CH, Hsiao YM, Lin CH, et al. Induction of premature senescence in human lung cancer by fungal immunomodulatory protein from Ganoderma tsugae[J]. Food Chem Toxicol, 2008, 46(5):1851-1859.
    [8] Cong WR, Xu H, Liu Y, et al. Production and functional characterization of a novel fungal immunomodulatory protein FIP-SN shuffled from two genes of Ganoderma species[J]. App Microbiol Biotechnol, 2014, 98(13):5967-5975.
    [9] 刘建文.抗肿瘤药理实验方法[M].北京:化学工业出版社,2010:29.
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    [13] Kino K, Yamashita A, Yamaoka K, et al. Isolation and characterization of a new immunomedulatory protein, ling zhi-8(LZ-8), from Ganoderma lucidum [J]. J Biol Chem, 1989, 264:472-478.
    [14] Lin WH, Huang CH, Hsu CI, et al. Dimerization of the N-terminal amphipathic alpha-helix domain of the fungal immunomodulatory protein from Ganoderma tsugae (Fip-gts) defined by a yeast two-hybrid system and site-directed mutagenesis[J]. J Biol Chem, 1997, 272(32):20044-20048.
    [15] Ishiyama M, Tominaga H, Shiga M, et al. Novel cell proliferation and cytotoxicity assays using a tetrazolium salt that produces a comparison of multiple cytotoxicity assays 61 water-soluble formazan dye[J]. In Vitro Toxicol, 1995, 8(2):187-190.
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Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

doi: 10.3969/j.issn.1006-0111.2016.03.008
  • 1. AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China;School of Life Sciences, Fudan University, Shanghai 200438, China
  • 2. AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China
  • 3. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China
  • Received Date: 2015-06-26
  • Rev Recd Date: 2015-10-21

Abstract: Objective To study the cell proliferative effects of fungal immunomodulatory proteins from Ganoderma spp. on 26 gastric cancer cell lines in vitro. Method 26 human gastric cancer cell lines were treated with FIPs by MTS assay. The average optical density(OD) in 490 nm and inhibition rate(GI50)was counted by Universal Microplate Spectrophotometer. Results Three FIPs showed similar profiling in 26 human gastric cancer cell lines after 72 h treatment in cell proliferation assay, which except for NUGC-4 and OCUM-1 did not showed obvious anti-proliferative effect, the other 24 human gastric cell lines showed some anti-proliferative effects, especially for 7 cell lines(NUGC-3, GTL-16, HGC-27, IM95m, SNU-638, SNU-216 and SNU-5) showing strong potency, with their GI50 less than 50 μg/ml. Conclusion FIPs showed strong anti-proliferative effects in some human gastric cancer cell lines in vitro, which had potential to be further developed as anti-gastric cancer drugs.

SHEN Liru, LI Ruihong, WANG Tailiang. Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
Citation: XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008
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