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Volume 34 Issue 3
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2016  >  34(3): 219-222,274

GAO Yanxia, SU Juan, JIN Huizi. Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 249-251,254. doi: 10.3969/j.issn.1006-0111.2016.03.014
Citation: JIANG Wenli, HUANG Caiguo. Wentilactone A inhibition of migration of small cell lung carcinoma NCI-H1688 cell line[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 219-222,274. doi: 10.3969/j.issn.1006-0111.2016.03.007
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Wentilactone A inhibition of migration of small cell lung carcinoma NCI-H1688 cell line

doi: 10.3969/j.issn.1006-0111.2016.03.007

  • Department of Biochemistry and Molecular Biology, Faculty of Basic Medicine, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2016-01-27
  • Rev Recd Date: 2016-03-31
  • Objective To investigate mechanism of Wentilactone A (WA) inhibition of small cell lung cancer(SCLC) cell line NCI-H1688 migration. Methods The migration and proliferation were analyzed by wounding-healing assay and MTT assay[3- (4, 5-Dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide, MTT]. Immunofluorescence was used to confirm the expression of ATF3 protein after WA treatment. Western blot was used to examine the expression of key proteins in ATF3/Nrf2/AKR1C1 signal pathway. Results WA inhibits the proliferation and migration of SCLC. MTT analysis showed WA inhibits the proliferation of NCI-H1688 cell line in a time-dependent manner. The number of migrated cells in WA treatment group was (8.73±1.06) mm, which was lower than that of control group (15.63±3.11) mm, The number of migrated cells in AKR1C1 expression group was (24.37±0.90) mm, the number of migrated cells in AKR1C1 expression and WA treatment group was (14.17±1.31) mm, with significant difference (P<0.05). WA enhances the nuclear expression of ATF3, and then reduces the expression of p-Nrf2 and AKR1C1. Conclusion WA inhibits the proliferation and migration of SCLC through ATF3/Nrf2/AKR1C1 signal pathway.
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    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 20.2 %其他: 20.2 %China: 1.4 %China: 1.4 %India: 0.4 %India: 0.4 %上海: 2.8 %上海: 2.8 %东莞: 0.4 %东莞: 0.4 %临汾: 0.4 %临汾: 0.4 %乌鲁木齐: 0.2 %乌鲁木齐: 0.2 %信阳: 0.2 %信阳: 0.2 %北京: 11.9 %北京: 11.9 %南京: 0.2 %南京: 0.2 %南宁: 0.2 %南宁: 0.2 %南昌: 0.5 %南昌: 0.5 %南通: 0.2 %南通: 0.2 %台湾省: 0.2 %台湾省: 0.2 %合肥: 0.2 %合肥: 0.2 %咸阳: 0.5 %咸阳: 0.5 %唐山: 1.2 %唐山: 1.2 %大连: 0.9 %大连: 0.9 %天津: 0.4 %天津: 0.4 %孝感: 0.2 %孝感: 0.2 %安顺: 0.2 %安顺: 0.2 %广州: 0.9 %广州: 0.9 %张家口: 0.7 %张家口: 0.7 %惠州: 0.2 %惠州: 0.2 %成都: 0.7 %成都: 0.7 %无锡: 0.2 %无锡: 0.2 %昆明: 0.2 %昆明: 0.2 %杭州: 0.4 %杭州: 0.4 %梧州: 0.2 %梧州: 0.2 %武汉: 0.5 %武汉: 0.5 %洛阳: 0.2 %洛阳: 0.2 %济南: 0.2 %济南: 0.2 %深圳: 0.7 %深圳: 0.7 %漳州: 0.2 %漳州: 0.2 %濮阳: 0.2 %濮阳: 0.2 %盐城: 0.4 %盐城: 0.4 %石家庄: 0.5 %石家庄: 0.5 %聊城: 0.2 %聊城: 0.2 %芒廷维尤: 26.2 %芒廷维尤: 26.2 %芝加哥: 0.2 %芝加哥: 0.2 %苏州: 0.5 %苏州: 0.5 %菏泽: 0.2 %菏泽: 0.2 %西宁: 20.5 %西宁: 20.5 %西安: 0.2 %西安: 0.2 %赤峰: 0.4 %赤峰: 0.4 %运城: 0.9 %运城: 0.9 %郑州: 0.2 %郑州: 0.2 %长沙: 0.4 %长沙: 0.4 %长治: 0.5 %长治: 0.5 %黄冈: 0.9 %黄冈: 0.9 %黄冈市麻城: 0.2 %黄冈市麻城: 0.2 %其他ChinaIndia上海东莞临汾乌鲁木齐信阳北京南京南宁南昌南通台湾省合肥咸阳唐山大连天津孝感安顺广州张家口惠州成都无锡昆明杭州梧州武汉洛阳济南深圳漳州濮阳盐城石家庄聊城芒廷维尤芝加哥苏州菏泽西宁西安赤峰运城郑州长沙长治黄冈黄冈市麻城Highcharts.com
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Wentilactone A inhibition of migration of small cell lung carcinoma NCI-H1688 cell line

doi: 10.3969/j.issn.1006-0111.2016.03.007
  • Department of Biochemistry and Molecular Biology, Faculty of Basic Medicine, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2016-01-27
  • Rev Recd Date: 2016-03-31

Abstract: Objective To investigate mechanism of Wentilactone A (WA) inhibition of small cell lung cancer(SCLC) cell line NCI-H1688 migration. Methods The migration and proliferation were analyzed by wounding-healing assay and MTT assay[3- (4, 5-Dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide, MTT]. Immunofluorescence was used to confirm the expression of ATF3 protein after WA treatment. Western blot was used to examine the expression of key proteins in ATF3/Nrf2/AKR1C1 signal pathway. Results WA inhibits the proliferation and migration of SCLC. MTT analysis showed WA inhibits the proliferation of NCI-H1688 cell line in a time-dependent manner. The number of migrated cells in WA treatment group was (8.73±1.06) mm, which was lower than that of control group (15.63±3.11) mm, The number of migrated cells in AKR1C1 expression group was (24.37±0.90) mm, the number of migrated cells in AKR1C1 expression and WA treatment group was (14.17±1.31) mm, with significant difference (P<0.05). WA enhances the nuclear expression of ATF3, and then reduces the expression of p-Nrf2 and AKR1C1. Conclusion WA inhibits the proliferation and migration of SCLC through ATF3/Nrf2/AKR1C1 signal pathway.

GAO Yanxia, SU Juan, JIN Huizi. Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 249-251,254. doi: 10.3969/j.issn.1006-0111.2016.03.014
Citation: JIANG Wenli, HUANG Caiguo. Wentilactone A inhibition of migration of small cell lung carcinoma NCI-H1688 cell line[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 219-222,274. doi: 10.3969/j.issn.1006-0111.2016.03.007
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