Genotoxicity evaluation of triptolide

TIAN Yijun; ZHENG Yiwen; ZHU Yuping; ZHANG Xiaofang; ZONG Ying; LU Guocai

doi:10.3969/j.issn.1006-0111.2016.03.006

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Objective To study the genotoxicity of triptolide, an important active component of Tripterygium wilfordii Hook f. Methods Ames test, in vitro chromosomal aberration test of CHO cell and in vivo micronucleus assay were performed to investigate the genotoxicity of triptolide. Results The Ames test showed that triptolide did not increase mutagenicity for TA97, TA98, TA100, TA102 and TA1535 strains at the dosage of 1.6~1000 μg per plate with and without metabolic activation system S9. Results of in vitro CHO cell chromosomal aberration test indicated that there was no statistical difference between the triptolide groups (doses of 0.01, 0.02 and 0.04 μg/ml) and the solvent control group with and without metabolic activation system S9. However, triptolide significantly increased polychromatophilic erythrocyte micronucleus formation at the dosage of 720 μg/kg in ICR mice. Conclusion Triptolide did not induce genetic toxicity based on the Ames test and chromosomal aberration test, but could increase micronucleus formation at the dosage of 720 μg/kg. These results indicated that triptolide may have potential genotoxicity on human health.

Genotoxicity evaluation of triptolide

Department of Hygiene and Toxicology, Second Military Medical University, Shanghai 200433, China

Received Date: 2015-11-15

Rev Recd Date: 2016-01-14

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Abstract: Objective To study the genotoxicity of triptolide, an important active component of Tripterygium wilfordii Hook f. Methods Ames test, in vitro chromosomal aberration test of CHO cell and in vivo micronucleus assay were performed to investigate the genotoxicity of triptolide. Results The Ames test showed that triptolide did not increase mutagenicity for TA97, TA98, TA100, TA102 and TA1535 strains at the dosage of 1.6~1000 μg per plate with and without metabolic activation system S9. Results of in vitro CHO cell chromosomal aberration test indicated that there was no statistical difference between the triptolide groups (doses of 0.01, 0.02 and 0.04 μg/ml) and the solvent control group with and without metabolic activation system S9. However, triptolide significantly increased polychromatophilic erythrocyte micronucleus formation at the dosage of 720 μg/kg in ICR mice. Conclusion Triptolide did not induce genetic toxicity based on the Ames test and chromosomal aberration test, but could increase micronucleus formation at the dosage of 720 μg/kg. These results indicated that triptolide may have potential genotoxicity on human health.

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Genotoxicity evaluation of triptolide

doi: 10.3969/j.issn.1006-0111.2016.03.006

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