Sensitized cytotoxic effects of dexamethasone or radiation on multiple myeloma cells by chloroquine

FU Hui; TAO Yulong; NI Min; LI Dongjie; SHEN Fuming

doi:10.3969/j.issn.1006-0111.2016.03.005

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Objective To investigate the role and possible mechanism of combination use of chloroquine (CQ) with either dexamethasone (DEX) or radiation on multiple myeloma (MM) cell line U266. Methods Cell viability of U266 treated with CQ alone, or CQ combined with either DEX or radiation was measured by cell counting kit-8(cck8). CalcuSyn method was used to assess effect of drugs interaction. Cell viability and apoptosis of U266 pre-treated with CQ were also measured by cck8 and flow cytometry after radiation. Expression of B-cellymphoma-2(Bcl-2) in U266 cells treated by CQ combined with DEX or radiation was determined by Western blot analysis. Results Either CQ or DEX displayed a dose dependent cell proliferation inhibitory effect on U266 cells. Cytotoxic effect of DEX (125 μmol/L) on U266 cells was enhanced and expression of Bcl-2 protein in U266 cells was decreased by combining with CQ (3.9 μmol/L). U266 cells were sensitized to radiation and cell death was induced by CQ (1.0 μmol/L). Conclusion CQ could sensitize cytotoxic effect of DEX or radiation on U266 cells, and the former was possibly related to down-regulation of Bcl-2 protein.

Sensitized cytotoxic effects of dexamethasone or radiation on multiple myeloma cells by chloroquine

1. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. Department of Pharmacy, Changzheng Hospital Affiliated to Second Military Medical University, Shanghai 200433, China

3. Department of Pharmacy, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China

4. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;Department of Pharmacy, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China

Received Date: 2016-03-09

Rev Recd Date: 2016-04-14

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Abstract: Objective To investigate the role and possible mechanism of combination use of chloroquine (CQ) with either dexamethasone (DEX) or radiation on multiple myeloma (MM) cell line U266. Methods Cell viability of U266 treated with CQ alone, or CQ combined with either DEX or radiation was measured by cell counting kit-8(cck8). CalcuSyn method was used to assess effect of drugs interaction. Cell viability and apoptosis of U266 pre-treated with CQ were also measured by cck8 and flow cytometry after radiation. Expression of B-cellymphoma-2(Bcl-2) in U266 cells treated by CQ combined with DEX or radiation was determined by Western blot analysis. Results Either CQ or DEX displayed a dose dependent cell proliferation inhibitory effect on U266 cells. Cytotoxic effect of DEX (125 μmol/L) on U266 cells was enhanced and expression of Bcl-2 protein in U266 cells was decreased by combining with CQ (3.9 μmol/L). U266 cells were sensitized to radiation and cell death was induced by CQ (1.0 μmol/L). Conclusion CQ could sensitize cytotoxic effect of DEX or radiation on U266 cells, and the former was possibly related to down-regulation of Bcl-2 protein.

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Reference (15)

[1]	Feng W, Fu Y, Zhang Y, et al. Establishment of stable multiple myeloma cell line with overexpressed PDCD5 and its proapoptosis mechanism[J]. Int J Clin Exp Pathol, 2015,8:10635-10643.
[2]	Dong H, Carlton ME, Lerner A, et al. Effect of cAMP signaling on expression of glucocorticoid receptor, Bim and Bad in glucocorticoid-sensitive and resistant leukemic and multiple myeloma cells[J]. Front Pharmacol, 2015,6:230.
[3]	Solomon VR, Lee H. Chloroquine and its analogs:a new promise of an old drug for effective and safe cancer therapies[J]. Eur J Pharmacol, 2009,625:220-233.
[4]	Fukuda T, Oda K, Wada-Hiraike O, et al. The anti-malarial chloroquine suppresses proliferation and overcomes cisplatin resistance of endometrial cancer cells via autophagy inhibition[J]. Gynecol Oncol, 2015,137:538-545.
[5]	Hu T, Li P, Luo Z, et al. Chloroquine inhibits hepatocellular carcinoma cell growth in vitro and in vivo[J]. Oncol Rep, 2015,35:43-49.
[6]	Tang MC, Wu MY, Hwang MH, et al. Chloroquine enhances gefitinib cytotoxicity in gefitinib-resistant nonsmall cell lung cancer cells[J]. PLoS One, 2015,10:e0119135.
[7]	Han MW, Lee JC, Choi JY, et al. Autophagy inhibition can overcome radioresistance in breast cancer cells through suppression of TAK1 activation[J]. Anticancer Res, 2014,34:1449-1455.
[8]	Ghafouri-Fard S, Seifi-Alan M, Shamsi R, et al. Immunotherapy in multiple myeloma using cancer-testis antigens[J]. Iran J Cancer Prev, 2015,8:e3755.
[9]	Lambert KE, Huang H, Mythreye K, et al. The type Ⅲ transforming growth factor-beta receptor inhibits proliferation, migration, and adhesion in human myeloma cells[J]. Mol Biol Cell, 2011,22:1463-1472.
[10]	刘竞,李昕,桂嵘,等. 程序化细胞死亡分子-5蛋白对地塞米松诱导的多发性骨髓瘤细胞凋亡的影响[J]. 中国老年学杂志, 2010,30:1972-1974.
[11]	Lee SW, Kim HK, Lee NH, et al. The synergistic effect of combination temozolomide and chloroquine treatment is dependent on autophagy formation and p53 status in glioma cells[J]. Cancer Lett, 2015,360:195-204.
[12]	Chou TC. Drug combination studies and their synergy quantification using the Chou-Talalay method[J]. Cancer Res, 2010,70:440-446.
[13]	袁晓丽,李涛,黄建鸣,等. 氯喹对食管癌细胞系放射增敏的实验研究[J]. 中国放射医学与防护杂志, 2014,34:823-826.
[14]	MS Ola MN, Ahsan H. Role of Bcl-2 family proteins and caspases in the regulation of apoptosis[J]. Mol Cell Biochem, 2011,351:41-58.
[15]	Niizuma K, Yoshioka H, Chen H, et al. Mitochondrial and apoptotic neuronal death signaling pathways in cerebral ischemia[J]. Biochim Biophys Acta, 2010,1802:92-99.

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Sensitized cytotoxic effects of dexamethasone or radiation on multiple myeloma cells by chloroquine

doi: 10.3969/j.issn.1006-0111.2016.03.005

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