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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2014  >  32(6): 444-447

LOU Yuefen, ZHU Quangang, YOU Benming, LI Jie, GU Xiaoyan, ZHANG Ruoxi. Study on local pharmacokinetics of baicalin gel after transdermal administration in rats by skin microdialysis in vivo[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(6): 444-447. doi: 10.3969/j.issn.1006-0111.2014.06.011
Citation: LOU Yuefen, ZHU Quangang, YOU Benming, LI Jie, GU Xiaoyan, ZHANG Ruoxi. Study on local pharmacokinetics of baicalin gel after transdermal administration in rats by skin microdialysis in vivo[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(6): 444-447. doi: 10.3969/j.issn.1006-0111.2014.06.011
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Study on local pharmacokinetics of baicalin gel after transdermal administration in rats by skin microdialysis in vivo

doi: 10.3969/j.issn.1006-0111.2014.06.011
  • 1.

    Department of Pharmacy, Branch of Shanghai First People's Hospital, Shanghai 200081, China

  • 2.

    Department of Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Pharmacy, Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433, China

  • 3.

    Department of Pharmacy, Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433, China

  • 4.

    Department of Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China

  • Received Date: 2014-01-02
  • Rev Recd Date: 2014-09-03
  • Objective To develop a novel skin microdialysis technology in vivo,and to determine the pharmacokinetic of baicalin after transdermal administration in rats. Methods An HPLC-MS/MS method used for the determination of baicalin in skin microdialysis samples was established,SD rats were pretreated with skin microdialysis operation under anesthesia, and then the baicalin gel was applied to the skin surface of probe in vivo. The baicalin concentration of skin microdialysates was determined, the time curve of baicalin concentration was drawn and the topical pharmacokinetics parameters of percutaneous absorption was calculated. Results Baicalin was optimized at the transitions m/z 447.3→271.2. The linearity correlation was good and the assay exhibited good precision and accuracy. The subcutaneous probe recovery of baicalin in vivo was(24.40±0.91)% and was stable over the 240 min study period. Baicalin could be detected in the microdialysis samples after transdermal administration, and its concentration continued to rise in 8 h. AUC0-t in skin tissue was(50.04±34.17) mg·min·L-1. Conclusion The method of skin microdialysis in vivo could be used in the local pharmacokinetic research of baicalin.
  • [1] 王 雷,王学艳,周雪琴,等. 黄芩苷凝胶设计与体外透皮性能的研究[J]. 中草药,2008,39(10):1502-1504.
    [2] 陈 芳,胡晋红,朱全刚. 用微透析法测定氢溴酸东茛菪碱凝胶经皮给药大鼠体内药动学研究[J]. 药学服务与研究,2012,12(4):270-273.
    [3] 李 洁,朱全刚,尤本明,等. 采用微透析联用HPLC法测定透析液中黄芩苷的浓度[J]. 药学服务与研究,2012,12(1):73-74.
    [4] Xing J, Chen X, Zhong D. Stability of baicalin in biological fluids in vitro[J]. J Pharm Biomed Anal, 2005,39(3-4):593-600.
    [5] Huang H, Zhang Y, Yang R,et al. Determination of baicalin in rat cerebrospinal fluid and blood using microdialysis coupled with ultra-performance liquid chromatography-tandem mass spectrometry[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2008,874(1-2):77-83.
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Study on local pharmacokinetics of baicalin gel after transdermal administration in rats by skin microdialysis in vivo

doi: 10.3969/j.issn.1006-0111.2014.06.011
  • 1. Department of Pharmacy, Branch of Shanghai First People's Hospital, Shanghai 200081, China
  • 2. Department of Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China;Department of Pharmacy, Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433, China
  • 3. Department of Pharmacy, Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433, China
  • 4. Department of Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received Date: 2014-01-02
  • Rev Recd Date: 2014-09-03

Abstract: Objective To develop a novel skin microdialysis technology in vivo,and to determine the pharmacokinetic of baicalin after transdermal administration in rats. Methods An HPLC-MS/MS method used for the determination of baicalin in skin microdialysis samples was established,SD rats were pretreated with skin microdialysis operation under anesthesia, and then the baicalin gel was applied to the skin surface of probe in vivo. The baicalin concentration of skin microdialysates was determined, the time curve of baicalin concentration was drawn and the topical pharmacokinetics parameters of percutaneous absorption was calculated. Results Baicalin was optimized at the transitions m/z 447.3→271.2. The linearity correlation was good and the assay exhibited good precision and accuracy. The subcutaneous probe recovery of baicalin in vivo was(24.40±0.91)% and was stable over the 240 min study period. Baicalin could be detected in the microdialysis samples after transdermal administration, and its concentration continued to rise in 8 h. AUC0-t in skin tissue was(50.04±34.17) mg·min·L-1. Conclusion The method of skin microdialysis in vivo could be used in the local pharmacokinetic research of baicalin.

LOU Yuefen, ZHU Quangang, YOU Benming, LI Jie, GU Xiaoyan, ZHANG Ruoxi. Study on local pharmacokinetics of baicalin gel after transdermal administration in rats by skin microdialysis in vivo[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(6): 444-447. doi: 10.3969/j.issn.1006-0111.2014.06.011
Citation: LOU Yuefen, ZHU Quangang, YOU Benming, LI Jie, GU Xiaoyan, ZHANG Ruoxi. Study on local pharmacokinetics of baicalin gel after transdermal administration in rats by skin microdialysis in vivo[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(6): 444-447. doi: 10.3969/j.issn.1006-0111.2014.06.011
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