Preparation and properties of alkalized shikonin liposome
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摘要: 目的 研究紫草素钠脂质体的制备工艺并考察其性质，旨在开发一种安全高效的紫草新型制剂。 方法 利用碱溶酸提法纯化紫草提取物，并采用乙醇注入法制备紫草素钠脂质体，测定其形貌、粒径和Zeta电位。利用高效液相建立左旋紫草素含量测定方法，并考察了脂质体包封率和体外释放性能。 结果 紫草素钠脂质体平均粒径为104.2 nm左右，且大小均一，成正态分布；Zeta电位为-14.8 mV；左旋紫草素在5~50 mg/L范围与峰面积呈良好的线性关系，相关系数为0.999 9；紫草素钠脂质体的包封率为43.67%，药物累积释放量为65.82%。 结论 成功制备了紫草素钠脂质体，具有较高的包封率和体外释放率。Abstract: Objective To study the preparation and properties of alkalized shikonin liposome. Methods Alkalized shikonin was purified from extracts of radix lithospermi with an alkali solution and acid formulation, and then the alkalized shikonin liposome was prepared by ethanol injection.The morphology, particle size and Zeta potential were determined, and the encapsulation efficiency and the in vitro release property were investigated.The content of L-shikonin was also measured by High Performance Liquid Chromatography. Results Alkalized shikonin liposome presented even sphere and normal distribution with particle size of 104.2 nm.The Zeta potential was-14.8 mV.L-shikonin was linear in the range of 5-50 mg/L with the correlation coefficient 0.999 9.The encapsulation efficiency was 43.67%.The final release rate was 65.82%. Conclusion Alkalized shikonin liposome was prepared successfully with good entrapment efficiency and in vitro release property.
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Key words:
- liposome /
- radixlithospermi /
- L-shikonin /
- in vitro release property
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