积雪草酸对颅脑损伤的保护作用及其机制研究

顾申勇; 朱秋珍; 王静; 刘玉兰; 张悦; 芮耀诚; 章越凡; 李铁军

doi:10.3969/j.issn.1006-0111.2016.03.004

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积雪草酸对颅脑损伤的保护作用及其机制研究

顾申勇, 朱秋珍, 王静, 刘玉兰, 张悦, 芮耀诚, 章越凡, 李铁军

文章导航 > 药学实践与服务 > 2016 > 34(3): 206-209,288

顾申勇, 朱秋珍, 王静, 刘玉兰, 张悦, 芮耀诚, 章越凡, 李铁军. 积雪草酸对颅脑损伤的保护作用及其机制研究[J]. 药学实践与服务, 2016, 34(3): 206-209,288. doi: 10.3969/j.issn.1006-0111.2016.03.004

引用本文:

GU Shenyong, ZHU Qiuzhen, WANG Jing, LIU Yulan, ZHANG Yue, RUI Yaocheng, ZHANG Yuefan, LI Tiejun. Protective effects and mechanism of Asiatic Acid on traumatic brain injury[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 206-209,288. doi: 10.3969/j.issn.1006-0111.2016.03.004

Citation:

GU Shenyong, ZHU Qiuzhen, WANG Jing, LIU Yulan, ZHANG Yue, RUI Yaocheng, ZHANG Yuefan, LI Tiejun. Protective effects and mechanism of Asiatic Acid on traumatic brain injury[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 206-209,288. doi: 10.3969/j.issn.1006-0111.2016.03.004

积雪草酸对颅脑损伤的保护作用及其机制研究

doi: 10.3969/j.issn.1006-0111.2016.03.004

1.
第二军医大学药学院药理学教研室, 上海 200433;复旦大学附属中山医院青浦分院药剂科, 上海 201700
2.
第二军医大学药学院药理学教研室, 上海 200433
3.
安徽中医药大学药学院, 安徽合肥 230031

基金项目: 上海市青浦区卫生和计划生育委员会科研课题(W2015-02)

Protective effects and mechanism of Asiatic Acid on traumatic brain injury

1.
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;Department of Pharmacy, Zhongshan Hospital Qingpu Branch Affiliated to Fudan University, Shanghai 201700, China
2.
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
3.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230031, China

摘要: 目的研究积雪草酸对颅脑损伤的保护作用及其机制。方法采用落重法建立大鼠闭合性颅脑损伤模型和液压冲击颅脑损伤模型,测定大鼠脑组织含水量、血脑屏障通透性、以及观察脑组织病理形态学变化,研究积雪草酸对颅脑损伤的保护作用;用酶联免疫吸附测定(ELISA)和聚合酶链式反应(PCR)检测炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)的表达,探讨其作用机制。结果积雪草酸可显著减少颅脑损伤后脑组织含水量,减轻血脑屏障通透性,改善脑组织细胞形态;并且可以显著抑制颅脑损伤后炎症因子TNF-α、IL-1β、IL-6的表达。结论积雪草酸具有保护颅脑创伤的作用,其作用机制可能与其抑制炎症作用有关。
- 积雪草酸 /
- 颅脑损伤 /
- 炎症
Abstract: Objective To study the protective effects and mechanism of Asiatic Acid (AA) on traumatic brain injury. Methods The closed craniocerebral injury model made by the weight-drop method and fluid percussion brain injury model were used in the study. Brain water content, blood brain barrier permeability and brain tissue pathology morphology were measured to study the neuroprotective effect of AA on traumatic brain injury. Moreover, the inflammatory cytokines such as TNF-α, IL-β, IL-6 were examined by PCR and ELISA to study the mechanism of AA. Results Brain water content and permeability of blood brain barrier were significantly decreased, the traumatic brain injury was nameliorated by AA. Moreover, the expressions of inflammatory cytokines TNF-α, IL-β, IL-6 were inhibited by AA. Conclusion AA has the protective effects on traumatic brain injury and the mechanism is related to the anti-inflammatory effect.
- Asiatic Acid /
- traumatic brain injury /
- inflammatory cytokines

[1]	Tweedie D, Rachmany L, Kim DS,et al. Mild traumatic brain injury-induced hippocampal gene expressions:The identification of target cellular processes for drug development[J]. J Neurosci Methods,2016,S0165-0270(16)00055-8.
[2]	赵宇新,李曼玲,冯伟红,等.积雪草的研究进展[J].中国中医药信息杂志,2002,9(8):81-84.
[3]	陈军,华维一,孙宏斌.积雪草酸及其衍生物的生物活性研究概况[J]. 中草药,2006,37(3):458-460.
[4]	Li TJ,Li B,Qin Y,et al.The application of asiatic acid and its derivatives in the preparation of antidepressants[P]. CN,1543964.2004-11-10.
[5]	Kim SR,Koo KA,Lee MK,et al. Asiatic acid derivatives enhance cognitive performance partly by improving acetylcholinesynthesis[J]. J Pharm Pharmacol,2004,56(10):1275-1282.
[6]	Cui FG. The application of asiatic acid and its derivatives in treatment of cardio-cerebrovasculardisease[P]. CN,1582946. 2005-02-23.
[7]	谭福源,李耀华.闭合性机械脑损伤实验动物模型[J].国外医学神经病学神经外科学分册,1994,21(2):80-82.
[8]	方加胜,哀贤瑞,刘运生. 实验性大鼠液压分级颅脑损伤模型的建立与研究[J].湖南医科大学学报,1996,21(5):385-389.
[9]	纪冬,辛绍杰.实时荧光定量PCR的发展和数据分析[J]. 生物技术通讯,2009,4(20):598-600.
[10]	Savitz SI, Cox CS Jr. Concise review:cell therapies for stroke and traumatic brain injury:targeting microglia[J]. Stem Cells,2016,34(3):537-542.

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积雪草酸对颅脑损伤的保护作用及其机制研究

doi: 10.3969/j.issn.1006-0111.2016.03.004

1. 第二军医大学药学院药理学教研室, 上海 200433;复旦大学附属中山医院青浦分院药剂科, 上海 201700

2. 第二军医大学药学院药理学教研室, 上海 200433

3. 安徽中医药大学药学院, 安徽合肥 230031

基金项目: 上海市青浦区卫生和计划生育委员会科研课题(W2015-02)

收稿日期: 2016-03-18
修回日期: 2016-04-01

关键词:

积雪草酸 /

颅脑损伤 /

炎症

摘要: 目的研究积雪草酸对颅脑损伤的保护作用及其机制。方法采用落重法建立大鼠闭合性颅脑损伤模型和液压冲击颅脑损伤模型,测定大鼠脑组织含水量、血脑屏障通透性、以及观察脑组织病理形态学变化,研究积雪草酸对颅脑损伤的保护作用;用酶联免疫吸附测定(ELISA)和聚合酶链式反应(PCR)检测炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)的表达,探讨其作用机制。结果积雪草酸可显著减少颅脑损伤后脑组织含水量,减轻血脑屏障通透性,改善脑组织细胞形态;并且可以显著抑制颅脑损伤后炎症因子TNF-α、IL-1β、IL-6的表达。结论积雪草酸具有保护颅脑创伤的作用,其作用机制可能与其抑制炎症作用有关。

English Abstract

Protective effects and mechanism of Asiatic Acid on traumatic brain injury

1. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;Department of Pharmacy, Zhongshan Hospital Qingpu Branch Affiliated to Fudan University, Shanghai 201700, China

2. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

3. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230031, China

Received Date: 2016-03-18
Rev Recd Date: 2016-04-01

Keywords:

Abstract: Objective To study the protective effects and mechanism of Asiatic Acid (AA) on traumatic brain injury. Methods The closed craniocerebral injury model made by the weight-drop method and fluid percussion brain injury model were used in the study. Brain water content, blood brain barrier permeability and brain tissue pathology morphology were measured to study the neuroprotective effect of AA on traumatic brain injury. Moreover, the inflammatory cytokines such as TNF-α, IL-β, IL-6 were examined by PCR and ELISA to study the mechanism of AA. Results Brain water content and permeability of blood brain barrier were significantly decreased, the traumatic brain injury was nameliorated by AA. Moreover, the expressions of inflammatory cytokines TNF-α, IL-β, IL-6 were inhibited by AA. Conclusion AA has the protective effects on traumatic brain injury and the mechanism is related to the anti-inflammatory effect.

引用本文:

Citation:

GU Shenyong, ZHU Qiuzhen, WANG Jing, LIU Yulan, ZHANG Yue, RUI Yaocheng, ZHANG Yuefan, LI Tiejun. Protective effects and mechanism of Asiatic Acid on traumatic brain injury[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 206-209,288. doi: 10.3969/j.issn.1006-0111.2016.03.004