Study on anti-microbial activity of allicin on campylobacter jejuni
-
摘要: 目的 研究大蒜素对空肠弯曲杆菌的抑菌作用和强度。 方法 采用纸片扩散法(K-B法)测定抑菌圈直径;采用肉汤稀释法分别测定大蒜素、环丙沙星、红霉素对空肠弯曲杆菌的最低抑菌浓度(MIC),比较大蒜素、环丙沙星、红霉素的最低抑菌浓度及抑菌率。 结果 大蒜素(MIC为12.80 μg/ml,抑菌率90.40%)对空肠弯曲杆菌的抑菌作用较环丙沙星(MIC为20.48 μg/ml,抑菌率90.21%)和红霉素(MIC为35.84 μg/ml,抑菌率90.33%)更强。 结论 大蒜素对空肠弯曲杆菌具有抑菌作用。Abstract: Objective To study the anti-microbial activity and strength of allicin on Campylobacter jejuni. Methods Disc diffusion method (K-B) was used to determine the diameter of the bacteriostatic ring. The minimal inhibitory concentration (MIC) of allicin, ciprofloxacin and erythromycin were detected by constant broth dilution method, respectively.The minimal inhibitory concentrations and bacteriostatic rate of allicin,ciprofloxacin and erythromycin were compared. Results The anti-microbial activity on Campylobacter jejuni of allicin (MIC 12.8 μg/ml, bactetiostatic rate 90.40%) was better than that of ciprofloxacin (MIC 20.48 μg/ml, bactetiostatic rate 90.21%) and erythromycin (MIC 35.84 μg/ml, bactetiostatic rate 90.33%). Conclusion Allicin has anti-microbial activity on Campylobacter jejuni in vitro.
-
Key words:
- allicin /
- ciprofloxacin /
- erythromycin /
- MIC /
- bacteriostatic rate
-
[1] Cavallito CJ,Bailey JH.Allicin,the antibacterial principle of Allium sativum. I. isolation,physicalproperties,and antibacterial action[J]. Am Chem Soc,1944,66(11):1950. [2] Lu X, Samuelson DR, Rasco BA, et al.Antimicrobial effect of diallyl sulphide on Campylobacter jejuni biofilms[J]. J Antimicrob Chemother,2012,67(8):1915-1926. [3] 骆海明,陈 倩.弯曲菌菌种冷冻保存及复苏方法研究[J]. 中国食品卫生杂志,2007,19(6):518. [4] 韩新锋,刘书亮,张晓利,等.鸡肉空肠弯曲杆菌的分离鉴定及耐药性分析[J]. 中国人兽共患病学报,2012,28(1):31. [5] Clinical and Laboratory Stanndards Institute.Approved standard M07-A8 methods for dilution antimicrobial susceptibiolity tests for bacteria that grow aerobically[S]. 8th ed.Villanova, PA:NCCLS,2009,29(2). [6] 孙长贵,曾向铭,杨 燕.肉汤稀释法酵母菌药物敏感性试验及质量控制介绍[J]. 江西医学检验,2007,25(1):57. [7] 陈振华,刘文恩. 碳青霉烯酶研究进展[J]. 国际检验医学杂志,2010,31(6):848. [8] 国家药典委员会.中华人民共和国药典2010年版一部[S]. 北京:中国医药科技出版社,2010:23.
计量
- 文章访问数: 2558
- HTML全文浏览量: 281
- PDF下载量: 112
- 被引次数: 0