摘要:
目的: 研究克糖特Ⅱ号对链脲霉素致糖尿病小鼠的降血糖作用并初步探讨其机制。 方法: 对小鼠腹腔注射链脲霉素(STZ)150 mg/kg建立糖尿病模型(DM),将建模成功的小鼠随机分为5组:格列本脲组(ig格列本脲50 mg/kg.d)、克糖特Ⅱ号高、中、低剂量(ig克糖特Ⅱ号分别相当于生药25 g/kg.d,12.5 g/kg.d,6.25 g/kg.d),糖尿病模型组(ig等体积生理盐水)和空白对照组(ig等体积生理盐水),连续给药15 d。并在相应时间采血测定空腹血糖(FBG)、胰岛素、血清肌酐(Cr)、尿酸(UA)、白蛋白(A lb)等指标。 结果: 克糖特Ⅱ号具有明显的降血糖作用,其作用与剂量呈正相关,并能提高胰岛素水平。同时能不同程度地降低血清Cr、UA(P<0.01),升高A lb(P<0.05)。 结论: 克糖特Ⅱ号能降低链脲霉素所致糖尿病小鼠的血糖,增加胰岛素的分泌,对糖尿病尤其并发症的治疗有潜在的应用价值。其降血糖作用机制可能与促进胰岛素分泌有关。
Abstract:
Objective: To investigate the hypoglycemic effects and its mechanism of KTTⅡ on streptozotocin(STZ)-induced diabetic mice. Methods: Diabetic model mice is established by injecting 150 mg/kg of STZ.These mice were divided into five groups randomly: model group,glibenclamide group(50 mg/kg·d,ig),high dose group of KTTⅡ(25 g/kg·d,ig),middle dose group of KTT(12.5 g/kg·d,ig) and low dose group of KTTⅡ(6.25 g/kg·d,ig),while the control and model group were administrated with the same volume of physiological saline for 15 days.During the experiment,the fasting blood glucose(FBG),insulin,creatinine(Cr),uric acid(UA),albumen(Alb) were measured at different time. Results: KTT Ⅱcould reduce the blood glucose concentration significantly in STZ-induced diabetic mice which was positively correlated with dosage-effect and time-effect.KTTⅡ could raise insulin level,and decrease Cr and UA levels in serum(P<0.01),increase Alb level in serum(P<0.05). Conclusion: The results suggested that KTTⅡ could decrease blood glucose,improve insulin level in STZ-induced diabetic mice,indicating that KTTⅡ may have a potential value for the treatment of DM and DM complications.The mechanism of reducing blood glucose is probably associated with promoting insulin secretion.
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