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Volume 29 Issue 5
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2011  >  29(5): 379-381,400

LI Mao-xing, ZHANG Chao, WU Lan, WEI Li-li, QIN Jian-guo, ZHANG Quan-long, JIA Zheng-ping. Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 379-381,400.
Citation: LI Mao-xing, ZHANG Chao, WU Lan, WEI Li-li, QIN Jian-guo, ZHANG Quan-long, JIA Zheng-ping. Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 379-381,400.
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Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC

  • 1.

    Department of Pharmacy, Lanzhou General Hospital of PLA, Key laboratory of the prevention and cure for the plateau environment damage, PLA, Lanzhou 730050, China;College of Life Science, Lanzhou University, Lanzhou 730000, China

  • 2.

    College of Chemical Engineering, Northwest University for Nationalities, Lanzhou 730030, China

  • 3.

    Department of Pharmacy, Lanzhou General Hospital of PLA, Key laboratory of the prevention and cure for the plateau environment damage, PLA, Lanzhou 730050, China

  • Received Date: 2011-02-23
  • Rev Recd Date: 2011-04-04
  • Objective To establish a HPLC method for the determination of three phenylethanoid glycosides in Phlomis younghusbandii. Methods HPLC analysis was performed on Symmetry C18 (4.6 mm×150 mm, 5 μm) column and the mixture of methanol-water (36:64, pH=4.5, HAc) was used as the mobile phase with a flow rate of 1.0 ml/min. The column temperature was 20 ℃ and the detection wavelength was 330 nm. Results The linear ranges of acteoside,alyssonoside,isoacteoside were 20~500(r=0.999 6), 10~130(r=0.999 5), 10~130 (r=0.999 7) μg/ml, respectively. The average recoveries (n=6) of them were all between 96% and 104%, RSD<5%. Conclusions This method was special, sensitive, accurate and with good repeatability, which could be used for quality control of Phlomis younghusbandii.
  • [1] 中国科学院中国植物志编辑委员会. 中国植物志( 65卷第二分册)[M]. 北京:化学工业出版社, 1995: 126,231.
    [2] 赵 斌, 董小萍, 吴健明. HPLC法测定螃蟹甲中山栀苷甲酯的含量[J]. 药物分析杂志,2009,29(2):323.
    [3] 张军莉, 徐 娟, 李茂星. HPLC法测定了藏药螃蟹甲中8-乙酰氧基山栀子苷甲酯的含量[J]. 解放军药学学报,2009,25(3):261.
    [4] 靖 会, 佐建锋, 李教社. 苯乙醇苷类化合物的药理研究进展[J]. 时珍国医国药, 2006, 17(3): 440.
    [5] 许敬英, 苏 奎, 周 静. 苯丙素苷类化合物的研究进展(Ⅱ)[J] .时珍国医国药, 2007, 18(7): 1770.
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Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC

  • 1. Department of Pharmacy, Lanzhou General Hospital of PLA, Key laboratory of the prevention and cure for the plateau environment damage, PLA, Lanzhou 730050, China;College of Life Science, Lanzhou University, Lanzhou 730000, China
  • 2. College of Chemical Engineering, Northwest University for Nationalities, Lanzhou 730030, China
  • 3. Department of Pharmacy, Lanzhou General Hospital of PLA, Key laboratory of the prevention and cure for the plateau environment damage, PLA, Lanzhou 730050, China
  • Received Date: 2011-02-23
  • Rev Recd Date: 2011-04-04

Abstract: Objective To establish a HPLC method for the determination of three phenylethanoid glycosides in Phlomis younghusbandii. Methods HPLC analysis was performed on Symmetry C18 (4.6 mm×150 mm, 5 μm) column and the mixture of methanol-water (36:64, pH=4.5, HAc) was used as the mobile phase with a flow rate of 1.0 ml/min. The column temperature was 20 ℃ and the detection wavelength was 330 nm. Results The linear ranges of acteoside,alyssonoside,isoacteoside were 20~500(r=0.999 6), 10~130(r=0.999 5), 10~130 (r=0.999 7) μg/ml, respectively. The average recoveries (n=6) of them were all between 96% and 104%, RSD<5%. Conclusions This method was special, sensitive, accurate and with good repeatability, which could be used for quality control of Phlomis younghusbandii.

LI Mao-xing, ZHANG Chao, WU Lan, WEI Li-li, QIN Jian-guo, ZHANG Quan-long, JIA Zheng-ping. Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 379-381,400.
Citation: LI Mao-xing, ZHANG Chao, WU Lan, WEI Li-li, QIN Jian-guo, ZHANG Quan-long, JIA Zheng-ping. Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 379-381,400.
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