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CUI Hai-zhen, LIU Fu-qiang, WANG Yan-ping, ZHAO Nan, LI Zi-yan. Studies of preparation and in vitro Absorption of ginkgo biloba leaf in-situ nasal gel[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 118-121.
Citation:
CHEN Yong, LIN Chen, ZHANG Jing, QIU Bin, ZHOU Xin, SONG Hong-tao. Determination of sirolimus in whole blood of beagle dog by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 353-355,375.
Determination of sirolimus in whole blood of beagle dog by UPLC-MS/MS
Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China;Fujian Medical College, Fuzhou 350101, China
2.
Fujian institute for Drug Control, Fuzhou 350025, China
3.
Deptment of Pharmacy, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, China
4.
Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China
Received Date: 2010-10-10
Rev Recd Date:
2011-03-31
Abstract
Objective To develop a UPLC-MS/MS method for the determination of sirolimus in whole blood of beagle dog. Methods Sirolimus and internal standard Tacrolimus were extracted from whole blood with liquid-liquid extraction,then separated on a Agilent C18 column (50 mm ×2.1 mm,1.7 μm) at the flow-rate of 0.30 ml/min. The mobile phase was Acetonitrile-water (90∶10) and the column temperature was 50 ℃. Electrospray ionization ( ESI) source was applied and operated in the positive ion mode. Multiple reaction monitoring (MRM) mode with the transitions of m/z 936.6→409.7 and m/z 826.6→415.4 were used to quantify sirolimus and the internal standard,respectively. Results The calibration linea range of blood-Sirolimu concentrations was 0.50~12.00 ng/ml(r=0.999 7).The low limit of quantification was 0.50 ng/ml . The intra-and inter-day precisions (RSDs) were less than 15%. The method recoveries were more than 90% . The extraction recoveries were more than 76%. Conclusions The method was specific,sensitive,rapid and suitable for the pharmacokinetic study of sirolimus in whole blood of beagle dog.
Yáñez JA,Forrest ML,Ohgami Y,et al. Pharmacometrics and delivery of novel nanoformulated PEG-b-poly (ε-cap-rolactone) micelles of rapamycin[J]. Cancer Chemoth Pharm,2008,61(1) : 133.
[3]
Ren B,Li SX,Deng B, et al. Determination of sirolimus in human whole blood by HPLC[J]. Chin Pham J,2004,39(1):52.
[4]
Lee JH,Cha KH,Cho W,et al. Quantitative determination of sirolimus in dog blood using liquid chromatography-tandem mass spectrometry, and its applications to pharmacokinetic studies[J].Journal of Pharmaceutical and Biomedical Analysis,2010,53(4):1042 .
Abstract: Objective To develop a UPLC-MS/MS method for the determination of sirolimus in whole blood of beagle dog. Methods Sirolimus and internal standard Tacrolimus were extracted from whole blood with liquid-liquid extraction,then separated on a Agilent C18 column (50 mm ×2.1 mm,1.7 μm) at the flow-rate of 0.30 ml/min. The mobile phase was Acetonitrile-water (90∶10) and the column temperature was 50 ℃. Electrospray ionization ( ESI) source was applied and operated in the positive ion mode. Multiple reaction monitoring (MRM) mode with the transitions of m/z 936.6→409.7 and m/z 826.6→415.4 were used to quantify sirolimus and the internal standard,respectively. Results The calibration linea range of blood-Sirolimu concentrations was 0.50~12.00 ng/ml(r=0.999 7).The low limit of quantification was 0.50 ng/ml . The intra-and inter-day precisions (RSDs) were less than 15%. The method recoveries were more than 90% . The extraction recoveries were more than 76%. Conclusions The method was specific,sensitive,rapid and suitable for the pharmacokinetic study of sirolimus in whole blood of beagle dog.
CUI Hai-zhen, LIU Fu-qiang, WANG Yan-ping, ZHAO Nan, LI Zi-yan. Studies of preparation and in vitro Absorption of ginkgo biloba leaf in-situ nasal gel[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 118-121.
Citation:
CHEN Yong, LIN Chen, ZHANG Jing, QIU Bin, ZHOU Xin, SONG Hong-tao. Determination of sirolimus in whole blood of beagle dog by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 353-355,375.
Yáñez JA,Forrest ML,Ohgami Y,et al. Pharmacometrics and delivery of novel nanoformulated PEG-b-poly (ε-cap-rolactone) micelles of rapamycin[J]. Cancer Chemoth Pharm,2008,61(1) : 133.
[3]
Ren B,Li SX,Deng B, et al. Determination of sirolimus in human whole blood by HPLC[J]. Chin Pham J,2004,39(1):52.
[4]
Lee JH,Cha KH,Cho W,et al. Quantitative determination of sirolimus in dog blood using liquid chromatography-tandem mass spectrometry, and its applications to pharmacokinetic studies[J].Journal of Pharmaceutical and Biomedical Analysis,2010,53(4):1042 .
CUI Hai-zhen, LIU Fu-qiang, WANG Yan-ping, ZHAO Nan, LI Zi-yan. Studies of preparation and in vitro Absorption of ginkgo biloba leaf in-situ nasal gel[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 118-121.
CUI Hai-zhen, LIU Fu-qiang, WANG Yan-ping, ZHAO Nan, LI Zi-yan. Studies of preparation and in vitro Absorption of ginkgo biloba leaf in-situ nasal gel[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 118-121.
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