CAO Yan, JING Jing, LV Di-ya, ZHU Zhen-yu, ZHANG Guo-qing, CHAI Yi-feng. Study on effective components from roots of Aconitum carmichaeli through cell membrane chromatography and HPLC-TOF/MS method[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 339-341,349.
| Citation:
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CAO Yan, JING Jing, LV Di-ya, ZHU Zhen-yu, ZHANG Guo-qing, CHAI Yi-feng. Study on effective components from roots of Aconitum carmichaeli through cell membrane chromatography and HPLC-TOF/MS method[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 339-341,349.
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Study on effective components from roots of Aconitum carmichaeli through cell membrane chromatography and HPLC-TOF/MS method
- 1.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- 2.
Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China
- Received Date: 2010-03-25
- Rev Recd Date:
2011-05-04
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Abstract
Objective To screen the effective components from roots of Aconitum carmichaeli by a cell membrane chromatography (CMC) combining with a HPLC-TOF/MS method. Methods Screening was conducted on a rat cardiac muscle CMC column, and mobile phase was 10 mmol/L phosphate buffer with a flow rate of 0.2 ml/min. Separation was conducted on a SHISEIDO CAPCELL PAK C18 column. The mobile phase consisted of 0.1% formic acid and acetonitrile using gradient elution with a flow rate of 1.0 ml/min. Identification was conducted by using TOF/MS in positive ion mode. Results Eight potential effective constituents were determined. Conclusion The combination of CMC and HPLC-TOF/MS method had provided a new way to discover active components targeting membrane receptors from natural products.
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