Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!
SUN Ji, CHEN Yan, LU Ying, ZHONG Yan-qiang. Study on transfection efficiency mediated by the complexes of lipoplexes and anionic liposomes[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(4): 265-268.
Citation:
SUN Ji, CHEN Yan, LU Ying, ZHONG Yan-qiang. Study on transfection efficiency mediated by the complexes of lipoplexes and anionic liposomes[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(4): 265-268.
Study on transfection efficiency mediated by the complexes of lipoplexes and anionic liposomes
School of Pharmacy, Second Military Medical University, Shanghai 200433,China
Received Date: 2011-01-04
Rev Recd Date:
2011-03-15
Abstract
Objective To evaluate the efficiency of different complexes of lipoplexes and anionic liposomes which mediated gene transfection to hepatoma cells(HepG2). Methods Cationic liposomes encapsulating report gene were prepared by reverse-evaporation method,which were pipetted into anionic liposomes to form complexes. The zeta potentials were measured by Zetasizer Nano ZS. Report gene expressing green fluorescent protein(GFP) was used in order to evaluate transfection efficiency of different complexes. MTT was used to measure the cytotoxicity. Results The anionic liposomes contributed to lower zeta potentials while could not effect the capability of pDNA load efficiency. The transfection efficiency of complexes was lower than that of cationic liposomes, however,the complexes presented higher cell viability. Conclusion In relation to the low cytotoxicity of the complexes, their transfection efficiency was relatively high.
Marika R, Markku T, Urtti A. Cell-surface glycosaminoglycans inhibited cation-mediated gene transfer[J]. J Gene Med, 2004,6(4):405.
[2]
Reinisalo M, Ruponen A, Urtti A. Polyplex-mediated gene transfer and cell cycle: effect of carrier on cellular uptake and intracellular kinetics and significance of glycosaminoglycans[J].J. Gene Med,2007,9(6):479.
[3]
Charudharshini S,Diane B. Optimization and characterization of anionic lipoplexes for gene delivery[J]. J. Controlled Release,2009,136(1):62.
[4]
Mignet N,Richard C,Scherman D,et al.Anionic pH-sensitive pegylated lipoplexes to deliver DNA to tumors[J]. Int J Pharm,2008,361(1-2):194.
[5]
Julia Lehtinen, et al. Glycosaminoglycan-resistant and pH-sensitive lipid-coated DNA complexes produced by detergent removal method[J]. J.Controlled Release, 2008,131(2):145.
Abstract: Objective To evaluate the efficiency of different complexes of lipoplexes and anionic liposomes which mediated gene transfection to hepatoma cells(HepG2). Methods Cationic liposomes encapsulating report gene were prepared by reverse-evaporation method,which were pipetted into anionic liposomes to form complexes. The zeta potentials were measured by Zetasizer Nano ZS. Report gene expressing green fluorescent protein(GFP) was used in order to evaluate transfection efficiency of different complexes. MTT was used to measure the cytotoxicity. Results The anionic liposomes contributed to lower zeta potentials while could not effect the capability of pDNA load efficiency. The transfection efficiency of complexes was lower than that of cationic liposomes, however,the complexes presented higher cell viability. Conclusion In relation to the low cytotoxicity of the complexes, their transfection efficiency was relatively high.
SUN Ji, CHEN Yan, LU Ying, ZHONG Yan-qiang. Study on transfection efficiency mediated by the complexes of lipoplexes and anionic liposomes[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(4): 265-268.
Citation:
SUN Ji, CHEN Yan, LU Ying, ZHONG Yan-qiang. Study on transfection efficiency mediated by the complexes of lipoplexes and anionic liposomes[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(4): 265-268.
Marika R, Markku T, Urtti A. Cell-surface glycosaminoglycans inhibited cation-mediated gene transfer[J]. J Gene Med, 2004,6(4):405.
[2]
Reinisalo M, Ruponen A, Urtti A. Polyplex-mediated gene transfer and cell cycle: effect of carrier on cellular uptake and intracellular kinetics and significance of glycosaminoglycans[J].J. Gene Med,2007,9(6):479.
[3]
Charudharshini S,Diane B. Optimization and characterization of anionic lipoplexes for gene delivery[J]. J. Controlled Release,2009,136(1):62.
[4]
Mignet N,Richard C,Scherman D,et al.Anionic pH-sensitive pegylated lipoplexes to deliver DNA to tumors[J]. Int J Pharm,2008,361(1-2):194.
[5]
Julia Lehtinen, et al. Glycosaminoglycan-resistant and pH-sensitive lipid-coated DNA complexes produced by detergent removal method[J]. J.Controlled Release, 2008,131(2):145.
DownLoad: