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Volume 29 Issue 1
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2011  >  29(1): 38-40

LIN Mei-yu, FU Wei, GONG Xiao-bin, LIN Jin-ming, WANG Xiao-yan. Determination of Tanshinone IIA by Fluorescence Spectrophotometry[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 38-40.
Citation: LIN Mei-yu, FU Wei, GONG Xiao-bin, LIN Jin-ming, WANG Xiao-yan. Determination of Tanshinone IIA by Fluorescence Spectrophotometry[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 38-40.
shu

Determination of Tanshinone IIA by Fluorescence Spectrophotometry

  • 1.

    Experiment Teaching Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • 2.

    Graduates Management Brigade, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2010-06-08
  • Rev Recd Date: 2010-07-08
  • Objective To study the fluorescence properties and analysis method of Tanshinone IIA. Methods Influencial factors including acidic media, the concentrations of substrate on the fluorescence intensity were studied. The stability of fluorescence of Tanshinone IIA was studied. Results Tanshinone IIA had good fluorescent characteristic in pH 2, which had an emission of fluorescence steadily under ultra-vioket radiation. Conclusion The method is simple, sensitive and could be suitable for the qualitative and quantitative analysis.
  • [1] 曾令杰,梁 晖,陈 悦,等.丹参酮IIA与丹酚酸B在丹参药材中的分布研究[J].现代中药研究与实践,2006, (2):7.
    [2] 杨天德,刘桥义,陶 军,等.丹参酮、纳洛酮对缺血再灌注心肌局部血流量的影响[J].中国药理学通报,1997,13(1):45.
    [3] 梁 勇,羊裔明,袁淑兰.丹参酮药理作用及临床应用研究进展[J].中草药, 2000,31(4):304.
    [4] 尉萍萍,赵 粼.HPLC测定丹参提取物中丹酚酸A含量[J].中国现代应用药学杂志,2008,25(3):241.
    [5] 徐韧柳,戴 敬.乌鸡白凤丸中丹参酮ⅡA的含量[J].中成药,1991,13(9):10.
    [6] 孙存济,白东鲁.丹参酮有关化合物的合成[J].药学学报,1985,20(1):39.
    [7] 谭生建,陈培让,王建社. RP-HPLC法测定抗栓保心片中丹参酮ⅡA的含量[J].中国中药杂志,1999,24(3):154.
    [8] 董 川,乔锦丽,杨 频.pH对隐丹参酮和丹参酮ⅡA的荧光特性及分子结构影响研究[J].光谱实验室,2000,17(4):369.
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Determination of Tanshinone IIA by Fluorescence Spectrophotometry

  • 1. Experiment Teaching Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • 2. Graduates Management Brigade, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2010-06-08
  • Rev Recd Date: 2010-07-08

Abstract: Objective To study the fluorescence properties and analysis method of Tanshinone IIA. Methods Influencial factors including acidic media, the concentrations of substrate on the fluorescence intensity were studied. The stability of fluorescence of Tanshinone IIA was studied. Results Tanshinone IIA had good fluorescent characteristic in pH 2, which had an emission of fluorescence steadily under ultra-vioket radiation. Conclusion The method is simple, sensitive and could be suitable for the qualitative and quantitative analysis.

LIN Mei-yu, FU Wei, GONG Xiao-bin, LIN Jin-ming, WANG Xiao-yan. Determination of Tanshinone IIA by Fluorescence Spectrophotometry[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 38-40.
Citation: LIN Mei-yu, FU Wei, GONG Xiao-bin, LIN Jin-ming, WANG Xiao-yan. Determination of Tanshinone IIA by Fluorescence Spectrophotometry[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 38-40.
shu

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