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ZHANG Wei, GAO Jing, ZHANG Min, WANG Xiao-Yu, HOU Xue-Mei, GAO Shen. Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 24-26,40.
Citation:
ZHANG Wei, GAO Jing, ZHANG Min, WANG Xiao-Yu, HOU Xue-Mei, GAO Shen. Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 24-26,40.
Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro
Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
2.
Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
Received Date: 2010-07-05
Rev Recd Date:
2010-12-17
Abstract
Objective To investigate the bio-efficacy of seven skin-lighteners on melanocytes in vitro. Methods After B-16 melanoma cells were treated with skin-lightener, the tyrosinase activity, the melanin content and cell viability were measured by utilization of L-Dopa as the substrate, colorimetric analysis, and MTT assay, respectively. Results each of the skin-lighteners showed a concentration-dependent reduction in tyrosinase activity and the melanin content and partially inhibited the cell viability. Conclusion all of the seven skin-lighteners could inhibit the melanocytes to produce melanin.
Petit L and Pierard GE. Skin lightening product revised[J]. Int. J. Cos. Sci., 2003, (25):169.
[5]
Maeda K, Fukuda M. Arbutin: Mechanism of its depigmenting action in melanocyte culture[J]. J Pharmacol Exp Ther, 1996, 276 (2): 765.
[6]
Virador VM, Kobayashi N, Matsunage J, et al. A standardized protocol for assessing regulators of pigmentation[J]. Anal Biochem, 1999, 270 (2): 207.
[7]
Jun SY, Park KM, Choi KW, et al. Inhibitory effects of arbutin-b-glycosides synthesized from enzymatic transglycosylation for melanogenesis[J]. Biotechnol Lett, 2008 (30): 743.
Zhong SM, Wu Y, SOO-MI Ahn, et al. Depigmentation of Melanocytes by the Treatment of Extracts from Traditional Chinese Herbs: a Cell Culture Assay[J].Biol. Pharm. Bull., 2006, 29(9): 1947.
[10]
Barry BW. Novel mechanisms and devices to enable successful transdermal drug delivery [J]. Eur. J. Pharm.Sci., 2001(14):101.
[11]
Verbaan FJ, Bal SM, Berg DJ van den, et al. Assembled microneedle arrays enhance the transport of compounds varying over a large range of molecular weight across human dermatomed skin[J]. J. Controlled Release, 2007(117): 238.
Abstract: Objective To investigate the bio-efficacy of seven skin-lighteners on melanocytes in vitro. Methods After B-16 melanoma cells were treated with skin-lightener, the tyrosinase activity, the melanin content and cell viability were measured by utilization of L-Dopa as the substrate, colorimetric analysis, and MTT assay, respectively. Results each of the skin-lighteners showed a concentration-dependent reduction in tyrosinase activity and the melanin content and partially inhibited the cell viability. Conclusion all of the seven skin-lighteners could inhibit the melanocytes to produce melanin.
ZHANG Wei, GAO Jing, ZHANG Min, WANG Xiao-Yu, HOU Xue-Mei, GAO Shen. Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 24-26,40.
Citation:
ZHANG Wei, GAO Jing, ZHANG Min, WANG Xiao-Yu, HOU Xue-Mei, GAO Shen. Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(1): 24-26,40.
Petit L and Pierard GE. Skin lightening product revised[J]. Int. J. Cos. Sci., 2003, (25):169.
[5]
Maeda K, Fukuda M. Arbutin: Mechanism of its depigmenting action in melanocyte culture[J]. J Pharmacol Exp Ther, 1996, 276 (2): 765.
[6]
Virador VM, Kobayashi N, Matsunage J, et al. A standardized protocol for assessing regulators of pigmentation[J]. Anal Biochem, 1999, 270 (2): 207.
[7]
Jun SY, Park KM, Choi KW, et al. Inhibitory effects of arbutin-b-glycosides synthesized from enzymatic transglycosylation for melanogenesis[J]. Biotechnol Lett, 2008 (30): 743.
Zhong SM, Wu Y, SOO-MI Ahn, et al. Depigmentation of Melanocytes by the Treatment of Extracts from Traditional Chinese Herbs: a Cell Culture Assay[J].Biol. Pharm. Bull., 2006, 29(9): 1947.
[10]
Barry BW. Novel mechanisms and devices to enable successful transdermal drug delivery [J]. Eur. J. Pharm.Sci., 2001(14):101.
[11]
Verbaan FJ, Bal SM, Berg DJ van den, et al. Assembled microneedle arrays enhance the transport of compounds varying over a large range of molecular weight across human dermatomed skin[J]. J. Controlled Release, 2007(117): 238.
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