Determination of polygalasaponin F in Polygala japonica by HPLC-ELSD method
- Received Date: 2009-10-22
- Rev Recd Date: 2009-12-14
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Key words:
- Polygalasaponin F /
- HPLC /
- ELSD
Abstract: Objective To establish an effective quality control method of the traditional Chinese medicine——Polygala japonica Houtt. Methods HPLC-ELSD method was adopted for the determination of polygalasaponin F in Polygala japonica Houtt.The analysis was performed on a SHISEIDO Capcell Pak C18(4.6 mm×250 mm,5 μm) as the analytical column,acetonitrile-water(3070,v/v) as the mobile phase.The flow rate was 1.0 ml/min.The column temperature was at 25 ℃. Results The validation was carried out and the linearity(r2 = 0.999 8) between 0.346 μg and 3.460 μg and recovery(99.85%) were acceptable.The precision and reproducibility were evaluated with RSD values 1.64% and 2.12%,respectively. Conclusion This method is simple and feasible for the determination of polygalasaponin F in Polygala japonica Houtt.
Citation: | JIAO Qing-qing, WANG Wei-yun, SU Juan, ZHANG Wei-dong. Determination of polygalasaponin F in Polygala japonica by HPLC-ELSD method[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 137-139. |