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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2010  >  28(2): 134-136

JING Jing, CAO Hong. Determination of auantion and uracil in Eupolyphaga seu Steleophaga by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 134-136.
Citation: JING Jing, CAO Hong. Determination of auantion and uracil in Eupolyphaga seu Steleophaga by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 134-136.
shu

Determination of auantion and uracil in Eupolyphaga seu Steleophaga by HPLC

  • 1.

    Department of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • 2.

    Institute for Drug and Instrument Control,Health Dept GLD of PLA,Beijing 100071,China

  • Received Date: 2009-09-06
  • Rev Recd Date: 2009-11-30
  • Objective To establish a method for determination of uracil and auantion in Eupolyphaga seu Steleophaga by HPLC. Methods The HPLC separation was performed on Alltima NH2(150 mm×4.6 mm,5 μm)column with a mixture of acetonitrile-water(9010)as the mobile phase,at a flow rate of 1.0 ml/min.Detection wavelength was at 205 nm,and column temperature was at 35 ℃. Results The linear response of Auantion and Uracil range from 0.404～3.23 μg(r=0.999 5,n=5)and 0.820～6.56 μg(r=0.999 5,n=5);the average recovery was 98.8% with RSD 0.96% and 99.0% with RSD 0.79% respectively(n=6). Conclusion The method is simple,accurate and suitable for the determination of auantion and uracil in Eupolyphaga seu Steleophaga.
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Determination of auantion and uracil in Eupolyphaga seu Steleophaga by HPLC

  • 1. Department of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • 2. Institute for Drug and Instrument Control,Health Dept GLD of PLA,Beijing 100071,China
  • Received Date: 2009-09-06
  • Rev Recd Date: 2009-11-30

Abstract: Objective To establish a method for determination of uracil and auantion in Eupolyphaga seu Steleophaga by HPLC. Methods The HPLC separation was performed on Alltima NH2(150 mm×4.6 mm,5 μm)column with a mixture of acetonitrile-water(9010)as the mobile phase,at a flow rate of 1.0 ml/min.Detection wavelength was at 205 nm,and column temperature was at 35 ℃. Results The linear response of Auantion and Uracil range from 0.404～3.23 μg(r=0.999 5,n=5)and 0.820～6.56 μg(r=0.999 5,n=5);the average recovery was 98.8% with RSD 0.96% and 99.0% with RSD 0.79% respectively(n=6). Conclusion The method is simple,accurate and suitable for the determination of auantion and uracil in Eupolyphaga seu Steleophaga.

JING Jing, CAO Hong. Determination of auantion and uracil in Eupolyphaga seu Steleophaga by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 134-136.
Citation: JING Jing, CAO Hong. Determination of auantion and uracil in Eupolyphaga seu Steleophaga by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(2): 134-136.
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