Study on inhibition of mimic peptides binding specifically with the first and second extra-cellular domain of CC chemokine receptor 5 on mice ear engorgement induced by dimethyl benzene

Key words: 
• CCR5 /
• mimic peptide /
• mce ear engorgement

Abstract: Objective To study the inhibition of mimic peptides binding specifically with the first and second extra-cellular domain of the CC chemokine receptor 5(CCR5) on mice ear engorgement induced by dimethyl benzene. Methods Phage display peptide library was used to screen peptide sequence bonding specifically with CCR5,then the model of mice ear engorgement was establish,and the four mimic peptides whose amino acid sequence are respectively STFTTTL(SL)、TPITQLL(TL)、SLPLPKP(SP)、QTSSAAL(QL) was synthesized,and the four peptides was injected into abdominal cavity of the ear engorgement model mice,take aspirin as the positive control of anti-inflammatory pharmacodynamics experiment.The left and the right mice ear tissue were obtained and weighted、HE stained,the four peptides was investigated the anti-inflammatory activity to mice ear engorgement induced by dimethyl benzene. Results After the left and the right mice ear tissue were weighted and HE stained,we find the four CCR5 mimic peptides had suppressed significantly mice ear engorgement induced by dimethyl benzene、hydrops and infiltrating of the inflammatory cells.The inhibition ratio is respectively 53.4%、47.3%、31.3%、25.2% that effect is significant(P<0.05). Conclusion All of the four short peptides respectively take the evident suppressing role on mice ear engorgement induced by dimethyl benzene.Thus this study indicated CCR5 may play a important role in the course of mice ear engorgement induced by dimethyl benzene.