Quantitative analysis of baicalin in Qinlan nasal drops by RP-HPLC

Key words: 
• RP-HPLC /
• Qinlan nasal drops /
• baicalin /
• content

Abstract: Objective To develop RP-HPLC method for the determination of baicalin in Qinlan nasal drops. Methods The chromatographic analysis was performed on Gemini C₁₈ 110A(4.60 mm×250 mm,5 μm)column.The mobile phase was consisted of methanol-0.2% phosphoric acid solution(50: 50).The flow rate was 0.8 ml/min.The detection wavelength was set at 277 nm,with injection volume 10 μl. Results A good linearity was obtained over the range of 709.4～7094.0μg/ml(r=0.999 9).The average recovery of baicalin was 99.6%,with RSD 1.33%(n=9). Conclusion The method is simple,accurate,specific and will be used for the quality control of Qinlan nasal drops.