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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2010  >  28(1): 25-28,69

YE Qi, WANG Jing, GUO Xue-qing, CHEN Chong-hong, ZHANG Jun-ping. Etoposide induces apoptosis via ubiquitin-proteasome pathway in HL-60 leukemia cells[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(1): 25-28,69.
Citation: YE Qi, WANG Jing, GUO Xue-qing, CHEN Chong-hong, ZHANG Jun-ping. Etoposide induces apoptosis via ubiquitin-proteasome pathway in HL-60 leukemia cells[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(1): 25-28,69.
shu

Etoposide induces apoptosis via ubiquitin-proteasome pathway in HL-60 leukemia cells

  • 1.

    Department of Pharmacology,Fujian Medical University,Fuzhou 350004,China;Department of Biomedical Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • 2.

    Department of Biomedical Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • 3.

    United Gene Holdings,Ltd.,Shanghai,200092,China

  • 4.

    Department of Pharmacology,Fujian Medical University,Fuzhou 350004,China

  • 5.

    Department of Biomedical Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • Received Date: 2009-09-17
  • Rev Recd Date: 2009-10-20
  • Objective To investigate the molecular mechanisms of Topoisomerase II inhibitor etopside(induced apoptosis on human leukemia HL-60 cells. Methods Cell growth and apoptosis were determined by MTT assay and flow cytometry.cDNA microarray was used to assess sensitive early gene expression profiles and Genmapp software was used to gene cluster and pathway analysis of the drug response. Results Etopside exhibited cell growth inhibition by 50 % at the concentrations of(30.17±0.26)mol/L and induced cell apoptosis from 4.38% to 53.96%.Genmapp analysis showed that etopside significantly inhibited ubiquitin-proteasome pathway(Z≥3.8) and decreased expression of genes involved in ubiquitin-proteasome pathway(PSMB5、PSMB7、PSMB8、PSMC3、PSMC5、RPN1and HLA(A). Conclusion Etoposide induces apoptosis via decreased expression of genes in ubiquitin-proteasome pathway.
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  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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Etoposide induces apoptosis via ubiquitin-proteasome pathway in HL-60 leukemia cells

  • 1. Department of Pharmacology,Fujian Medical University,Fuzhou 350004,China;Department of Biomedical Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • 2. Department of Biomedical Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • 3. United Gene Holdings,Ltd.,Shanghai,200092,China
  • 4. Department of Pharmacology,Fujian Medical University,Fuzhou 350004,China
  • 5. Department of Biomedical Pharmacy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • Received Date: 2009-09-17
  • Rev Recd Date: 2009-10-20

Abstract: Objective To investigate the molecular mechanisms of Topoisomerase II inhibitor etopside(induced apoptosis on human leukemia HL-60 cells. Methods Cell growth and apoptosis were determined by MTT assay and flow cytometry.cDNA microarray was used to assess sensitive early gene expression profiles and Genmapp software was used to gene cluster and pathway analysis of the drug response. Results Etopside exhibited cell growth inhibition by 50 % at the concentrations of(30.17±0.26)mol/L and induced cell apoptosis from 4.38% to 53.96%.Genmapp analysis showed that etopside significantly inhibited ubiquitin-proteasome pathway(Z≥3.8) and decreased expression of genes involved in ubiquitin-proteasome pathway(PSMB5、PSMB7、PSMB8、PSMC3、PSMC5、RPN1and HLA(A). Conclusion Etoposide induces apoptosis via decreased expression of genes in ubiquitin-proteasome pathway.

YE Qi, WANG Jing, GUO Xue-qing, CHEN Chong-hong, ZHANG Jun-ping. Etoposide induces apoptosis via ubiquitin-proteasome pathway in HL-60 leukemia cells[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(1): 25-28,69.
Citation: YE Qi, WANG Jing, GUO Xue-qing, CHEN Chong-hong, ZHANG Jun-ping. Etoposide induces apoptosis via ubiquitin-proteasome pathway in HL-60 leukemia cells[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(1): 25-28,69.
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