Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 28 Issue 1
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2010  >  28(1): 11-13,44

ZHOU Gui-chen, LIU Qing-feng, LU Ying, ZHONG Yan-qiang. Stability studies of lysozyme for preformulation[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(1): 11-13,44.
Citation: ZHOU Gui-chen, LIU Qing-feng, LU Ying, ZHONG Yan-qiang. Stability studies of lysozyme for preformulation[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(1): 11-13,44.
shu

Stability studies of lysozyme for preformulation

  • Department of Pharmaceutical Science,School of Pharmacy,Second Military Medical University,Shanghai 200433,China

  • Received Date: 2009-10-10
  • Rev Recd Date: 2009-11-30
  • Objective To investigate the influence of pH value,temperature,protectant and organic solvent on the physical stability and biological activity of lysozyme. Methods The concentration of lysozyme was determined by HPLC and the biological activity of lysozyme was tested by turbidimetric method. Results Lysozyme was physically stable at pH4.0 and pH10.0.The biological activity of lysozyme was kept well at pH4.0 while it was totally destroyed at pH1.0.Low temperature tended to protect lysozyme from damage.Mannitol protected lysozyme at the beginning and then accelerated its destruction.Organic solvent seriously destroyed lysozyme while the biological activity of lysozyme remained to some degree. Conclusion The pH value,temperature,protectant and organic solvent can obviously influence the physical stability and biological activity of lysozyme.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2545) PDF downloads(995) Cited by()

Related
Proportional views

Stability studies of lysozyme for preformulation

  • Department of Pharmaceutical Science,School of Pharmacy,Second Military Medical University,Shanghai 200433,China
  • Received Date: 2009-10-10
  • Rev Recd Date: 2009-11-30

Abstract: Objective To investigate the influence of pH value,temperature,protectant and organic solvent on the physical stability and biological activity of lysozyme. Methods The concentration of lysozyme was determined by HPLC and the biological activity of lysozyme was tested by turbidimetric method. Results Lysozyme was physically stable at pH4.0 and pH10.0.The biological activity of lysozyme was kept well at pH4.0 while it was totally destroyed at pH1.0.Low temperature tended to protect lysozyme from damage.Mannitol protected lysozyme at the beginning and then accelerated its destruction.Organic solvent seriously destroyed lysozyme while the biological activity of lysozyme remained to some degree. Conclusion The pH value,temperature,protectant and organic solvent can obviously influence the physical stability and biological activity of lysozyme.

ZHOU Gui-chen, LIU Qing-feng, LU Ying, ZHONG Yan-qiang. Stability studies of lysozyme for preformulation[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(1): 11-13,44.
Citation: ZHOU Gui-chen, LIU Qing-feng, LU Ying, ZHONG Yan-qiang. Stability studies of lysozyme for preformulation[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(1): 11-13,44.
shu

    HTML

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return