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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2009  >  27(4): 289-290,293

LIU Li, LONG Ping, YAO Jian-zhong. Determination of luteolin in three kinds of Chrysanthemum morifolium by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 289-290,293.
Citation: LIU Li, LONG Ping, YAO Jian-zhong. Determination of luteolin in three kinds of Chrysanthemum morifolium by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 289-290,293.
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Determination of luteolin in three kinds of Chrysanthemum morifolium by HPLC

  • 1.

    School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Center for Certification and Evaluation, Shanghai Food and Drug Administration, Shanghai 200020, China

  • 2.

    Department of Phylaxiology, College of Medicine, Shihezi University, Shihezi 832002, China

  • 3.

    School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2009-01-06
  • Objective :To determine the content of luteolin in three kinds of Chrysanthemum morifolium that are often seen in market by HPLC. Methods : The luteolin was quantified by reverse-HPLC on Diamosil C18 column(250 mm×4.6 mm,5 μm)using methanol-water(changed from 50∶50 to 80∶20 in 30 min at pace) as mobile phase with a flow rate of 1 mL/min.The detecting wavelength was 350 nm. Results :The linear range of luteolin was 0.224 ～2.24 μg(r=0.999 9).The average recovery was 99.40%,and RSD was 2.67%(n=9).The average content of luteolin in Hangju,Gongju and Haoju was 0.34,0.41,0.69 mg/g,and RSD was 0.8%,1.2%,1.3%,respectively.(n=6). Conclusion :The content of luteolin in Haoju was the highest.The established method is delicate,simple,convenient,accurate,reproducible,and suitable for the quality control of luteolin in defferent kinds of Chrysanthemum morifolium that are often seen in market.
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Determination of luteolin in three kinds of Chrysanthemum morifolium by HPLC

  • 1. School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Center for Certification and Evaluation, Shanghai Food and Drug Administration, Shanghai 200020, China
  • 2. Department of Phylaxiology, College of Medicine, Shihezi University, Shihezi 832002, China
  • 3. School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2009-01-06

Abstract: Objective :To determine the content of luteolin in three kinds of Chrysanthemum morifolium that are often seen in market by HPLC. Methods : The luteolin was quantified by reverse-HPLC on Diamosil C18 column(250 mm×4.6 mm,5 μm)using methanol-water(changed from 50∶50 to 80∶20 in 30 min at pace) as mobile phase with a flow rate of 1 mL/min.The detecting wavelength was 350 nm. Results :The linear range of luteolin was 0.224 ～2.24 μg(r=0.999 9).The average recovery was 99.40%,and RSD was 2.67%(n=9).The average content of luteolin in Hangju,Gongju and Haoju was 0.34,0.41,0.69 mg/g,and RSD was 0.8%,1.2%,1.3%,respectively.(n=6). Conclusion :The content of luteolin in Haoju was the highest.The established method is delicate,simple,convenient,accurate,reproducible,and suitable for the quality control of luteolin in defferent kinds of Chrysanthemum morifolium that are often seen in market.

LIU Li, LONG Ping, YAO Jian-zhong. Determination of luteolin in three kinds of Chrysanthemum morifolium by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 289-290,293.
Citation: LIU Li, LONG Ping, YAO Jian-zhong. Determination of luteolin in three kinds of Chrysanthemum morifolium by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 289-290,293.
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