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XIA Ai-jun, HUANG He-zhou, GAO Gang, ZHANG Hai, ZHU Zhen-yu, CHAI Yi-feng. Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 284-285,288.
Citation:
XIA Ai-jun, HUANG He-zhou, GAO Gang, ZHANG Hai, ZHU Zhen-yu, CHAI Yi-feng. Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 284-285,288.
Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC
Department of pharmacy, 303th Hospital of PLA, Nanning 530021, China
2.
School of pharmacy, Second Military Medical University, Shanghai 200433, China
3.
Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China
Received Date: 2008-11-20
Abstract
Objective : To establish a HPLC method for simultaneous determination of five flavone constituents in Epimedium Brevicornum. Methods :The determination was performed on Diamonsil-C18 column(4.6mm×250mm,5μm,Dikma).The mobile phase was consisted of acetonitrile-formic acid aqueous solution with a flow rate of 0.9 mL/min.The detection wavelength was set at 270 nm. Results :A method has been established for determination of epimedin A1,epimedin B,icariin,sagittatoside A,baohuoside I in one run by HPLC. Conclusion :The established method is simple,convenient,accurate and reliable,and suitable for the quality control of Epimedium Brevicornum.
Abstract: Objective : To establish a HPLC method for simultaneous determination of five flavone constituents in Epimedium Brevicornum. Methods :The determination was performed on Diamonsil-C18 column(4.6mm×250mm,5μm,Dikma).The mobile phase was consisted of acetonitrile-formic acid aqueous solution with a flow rate of 0.9 mL/min.The detection wavelength was set at 270 nm. Results :A method has been established for determination of epimedin A1,epimedin B,icariin,sagittatoside A,baohuoside I in one run by HPLC. Conclusion :The established method is simple,convenient,accurate and reliable,and suitable for the quality control of Epimedium Brevicornum.
XIA Ai-jun, HUANG He-zhou, GAO Gang, ZHANG Hai, ZHU Zhen-yu, CHAI Yi-feng. Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 284-285,288.
Citation:
XIA Ai-jun, HUANG He-zhou, GAO Gang, ZHANG Hai, ZHU Zhen-yu, CHAI Yi-feng. Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 284-285,288.
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