Simultaneous determination of chlorogenic acid and baicalin in Yinhuang oral liquid by HPLC

Key words: 
• HPLC /
• Yinhuang oral liquid /
• chlorgenic acid /
• baicalin

Abstract: Objective: To develop a HPLC quantitative method for the determination of chlorgenic acid and baicalin in Yinhuang Oral liquid. Methods: An Agilent C18 column(ZORBAX Eclipse XDB-C18,4.6×150 mm,5 μm)was used and a mixture of methanol-water-phosphorc acid(50:950:5)was used as mobile phase A,methanol as mobile phase B,gradient elution:0～30,A:95%～35%,B:5%～65%.The flow rate was 1.0 mL/min and monitored at 327 nm. Results: The retention time of chlorgenic acid and baicalin were 10.5 min and 23.8 min,resp...