Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!
NING Wei-wei, LIU Xue-fei, ZHANG Xiao-meng, ZHENG Can-hui, SHENG Chun-quan, ZHOU You-jun, ZHANG Ling, LV Jia-guo, ZHU Ju. Design,synthesis of novel quinazolinon compounds as human anti-acrosin inhibitor[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(4): 296-298,312.
Citation:
ZHANG Ya-ning, WU Bo, YANG Ji-gang, XU Xiu-ying, ZHENG Yi-min. Quantitative determination of luteolin in Pyrrosia lingua by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 359-361.
Quantitative determination of luteolin in Pyrrosia lingua by HPLC
Undergraduate of Grade 2003,College of Bioengineer,Chongqing Institue of Technology,Chongqing 400050,China
2.
Laboratory of Natural Drugs,College of Bioengineer,Chongqing Institue of Technology,Chongqing 400050,China
Received Date: 2005-07-03
Abstract
Objective: To determine the content of luteolin in Pyrrosia lingua by HPLC. Methods: The HPLC method was used to deter- mine the content of luteolin from Pyrrosia lingua.The analysis was carried out on Shimadzu C18 colunm（150mm×4.6mm,5μm）.The mobile phase was MeOH-0.2% H3 PO4（50:50）.Flow-rate was 1.0mL/min.Wave-length was 254nm.Temperature was room temperature. Results: The method was simple and had a good linear relationship.The liner range of luteolin is 0.40～2.00μg and gave a correlation （r）of 0.999 6.The recovery,was 95.85%,RSD 0.27%. Conclusion: The method was easy to determine the content of luteolin from Pyrrosia lingua.
Abstract: Objective: To determine the content of luteolin in Pyrrosia lingua by HPLC. Methods: The HPLC method was used to deter- mine the content of luteolin from Pyrrosia lingua.The analysis was carried out on Shimadzu C18 colunm（150mm×4.6mm,5μm）.The mobile phase was MeOH-0.2% H3 PO4（50:50）.Flow-rate was 1.0mL/min.Wave-length was 254nm.Temperature was room temperature. Results: The method was simple and had a good linear relationship.The liner range of luteolin is 0.40～2.00μg and gave a correlation （r）of 0.999 6.The recovery,was 95.85%,RSD 0.27%. Conclusion: The method was easy to determine the content of luteolin from Pyrrosia lingua.
NING Wei-wei, LIU Xue-fei, ZHANG Xiao-meng, ZHENG Can-hui, SHENG Chun-quan, ZHOU You-jun, ZHANG Ling, LV Jia-guo, ZHU Ju. Design,synthesis of novel quinazolinon compounds as human anti-acrosin inhibitor[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(4): 296-298,312.
Citation:
ZHANG Ya-ning, WU Bo, YANG Ji-gang, XU Xiu-ying, ZHENG Yi-min. Quantitative determination of luteolin in Pyrrosia lingua by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 359-361.
HTML
Catalog
HTML
Export File
Citation
NING Wei-wei, LIU Xue-fei, ZHANG Xiao-meng, ZHENG Can-hui, SHENG Chun-quan, ZHOU You-jun, ZHANG Ling, LV Jia-guo, ZHU Ju. Design,synthesis of novel quinazolinon compounds as human anti-acrosin inhibitor[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(4): 296-298,312.
NING Wei-wei, LIU Xue-fei, ZHANG Xiao-meng, ZHENG Can-hui, SHENG Chun-quan, ZHOU You-jun, ZHANG Ling, LV Jia-guo, ZHU Ju. Design,synthesis of novel quinazolinon compounds as human anti-acrosin inhibitor[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(4): 296-298,312.
DownLoad: