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Volume 24 Issue 6
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2006  >  (6): 359-361

ZHANG Ya-ning, WU Bo, YANG Ji-gang, XU Xiu-ying, ZHENG Yi-min. Quantitative determination of luteolin in Pyrrosia lingua by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 359-361.
Citation: ZHANG Ya-ning, WU Bo, YANG Ji-gang, XU Xiu-ying, ZHENG Yi-min. Quantitative determination of luteolin in Pyrrosia lingua by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 359-361.
shu

Quantitative determination of luteolin in Pyrrosia lingua by HPLC

  • 1.

    Undergraduate of Grade 2003,College of Bioengineer,Chongqing Institue of Technology,Chongqing 400050,China

  • 2.

    Laboratory of Natural Drugs,College of Bioengineer,Chongqing Institue of Technology,Chongqing 400050,China

  • Received Date: 2005-07-03
  • Objective: To determine the content of luteolin in Pyrrosia lingua by HPLC. Methods: The HPLC method was used to deter- mine the content of luteolin from Pyrrosia lingua.The analysis was carried out on Shimadzu C18 colunm（150mm×4.6mm,5μm）.The mobile phase was MeOH-0.2% H3 PO4（50:50）.Flow-rate was 1.0mL/min.Wave-length was 254nm.Temperature was room temperature. Results: The method was simple and had a good linear relationship.The liner range of luteolin is 0.40～2.00μg and gave a correlation （r）of 0.999 6.The recovery,was 95.85%,RSD 0.27%. Conclusion: The method was easy to determine the content of luteolin from Pyrrosia lingua.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Quantitative determination of luteolin in Pyrrosia lingua by HPLC

  • 1. Undergraduate of Grade 2003,College of Bioengineer,Chongqing Institue of Technology,Chongqing 400050,China
  • 2. Laboratory of Natural Drugs,College of Bioengineer,Chongqing Institue of Technology,Chongqing 400050,China
  • Received Date: 2005-07-03

Abstract: Objective: To determine the content of luteolin in Pyrrosia lingua by HPLC. Methods: The HPLC method was used to deter- mine the content of luteolin from Pyrrosia lingua.The analysis was carried out on Shimadzu C18 colunm（150mm×4.6mm,5μm）.The mobile phase was MeOH-0.2% H3 PO4（50:50）.Flow-rate was 1.0mL/min.Wave-length was 254nm.Temperature was room temperature. Results: The method was simple and had a good linear relationship.The liner range of luteolin is 0.40～2.00μg and gave a correlation （r）of 0.999 6.The recovery,was 95.85%,RSD 0.27%. Conclusion: The method was easy to determine the content of luteolin from Pyrrosia lingua.

ZHANG Ya-ning, WU Bo, YANG Ji-gang, XU Xiu-ying, ZHENG Yi-min. Quantitative determination of luteolin in Pyrrosia lingua by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 359-361.
Citation: ZHANG Ya-ning, WU Bo, YANG Ji-gang, XU Xiu-ying, ZHENG Yi-min. Quantitative determination of luteolin in Pyrrosia lingua by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2006, (6): 359-361.
shu

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