Effects of estrogen and tamoxifen on the expression of BP1 in MCF-7, a human breast carcinoma cell line

Key words: 
• MCF-7 cell /
• BP1 gene /
• apoptosis /
• in situ hybridization /
• bcl-2 /
• Immunohistochemistry

Abstract: Objective To investigate the espression of BP1 gene after estrogen and tamoxifen effected on breast cancer cell line MCF-7 respectively.Methods; With screened concentration of estrogen (10^-8 mol/L) and tamoxifen (10^-6 mol/L) by MTT assay treated with MCF-7 72 hours,then detected the expressions of BP1 mRNA by in situ hybridization method and bcl-2 protein by immuno-histoc-hemistry.Determined quantitatively apoptosis cells of MCF-7 by TUNEL method.Results The percents of BP1 mRNA espression of MCF-7 cells in E2 treated group and TAM treated group were (95.0%±3.94% ) and (58.5%±5.21% ), and those of bcl-2 protein were (80.9%±1.73%) and (37.8%±2.39%), howere, the expressions of BP1 and bcl-2 were (76.1%±6.54%) and (63.5%±3.28%) in control group respectively.There were all significant difference between each two groups (P<0.05 ).There was a significant difference in apoptosis index in either group treated with E2 (0.67±0.27) or TAM (6.7±0.76) in comparing with that of the control group (3.2±0.79) (P<0.05).Conclusion The expression of BP1 mRNA was possibly regulated by ER mediated signal transduction pathway in ER positive breast carcinoma cell line, and BP1 might play an important role in tumorgenesis of breast carcinoma through up-regulate espression mediated by E2 and apoptosis inhibition.BP1 might serve as a poor prognostic markers of breast carcinoma.