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LONG Xiao-hua, LI Hui, HUANG Rong. Determination of bererine in compound Sophora flavescens lotions by three variables complex chromatometry[J]. Journal of Pharmaceutical Practice and Service, 2004, (5): 294-296.
Citation:
LONG Xiao-hua, LI Hui, HUANG Rong. Determination of bererine in compound Sophora flavescens lotions by three variables complex chromatometry[J]. Journal of Pharmaceutical Practice and Service, 2004, (5): 294-296.
Determination of bererine in compound Sophora flavescens lotions by three variables complex chromatometry
The Fourth Hospital of Changsha, Changsha 410006, China
Received Date: 2003-11-17
Abstract
Objective To establish three variables complex chromometry for the determination of berberine in compound Sophora flavescens lotions. Methods The berberine formed three variables complex with bromophenol blue and quinine in pH6.7,then it was withdrawn with chloroform. The detection wavelength was 610nm. Results The linear range of berberine was the 0.8～4mg/L(r = 0.9999),the average recovery was 98.65%,RSD was 1.12%. Conclusion The method had a good sensitive, and reproducibility. It can be used for determination of berberine in compound sophora flavescens lotions.
Abstract: Objective To establish three variables complex chromometry for the determination of berberine in compound Sophora flavescens lotions. Methods The berberine formed three variables complex with bromophenol blue and quinine in pH6.7,then it was withdrawn with chloroform. The detection wavelength was 610nm. Results The linear range of berberine was the 0.8～4mg/L(r = 0.9999),the average recovery was 98.65%,RSD was 1.12%. Conclusion The method had a good sensitive, and reproducibility. It can be used for determination of berberine in compound sophora flavescens lotions.
LONG Xiao-hua, LI Hui, HUANG Rong. Determination of bererine in compound Sophora flavescens lotions by three variables complex chromatometry[J]. Journal of Pharmaceutical Practice and Service, 2004, (5): 294-296.
Citation:
LONG Xiao-hua, LI Hui, HUANG Rong. Determination of bererine in compound Sophora flavescens lotions by three variables complex chromatometry[J]. Journal of Pharmaceutical Practice and Service, 2004, (5): 294-296.
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