Studies on UV spectrophotometric determination and thinlayer chromatographic identification of tetracaine hydrochloride eye drop

A method of determination by UV spectrophotometry and identification by thin-layer chromatography of tetracaine hydrochloride eye drop was established. The maximum absorbance wavel ength at 310±1nm was selected for determination wavelength, ethyl hydroxybenzoate has no interferrence. The result showed that the absorbance coeffecient (E_1cm^1%) of tetracaine hydrochloride at 310±1nm was 760,RSD=0.5% (n=20), the mean recovery for sample was 99.5%, RSD=0.5% (n=7). This method is simple, rapid, accurate and reproducible.