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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2019  >  37(6): 537-542

GAO Yanxia, SU Juan, JIN Huizi. Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 249-251,254. doi: 10.3969/j.issn.1006-0111.2016.03.014
Citation: WANG Guiling, FANG Jianqiang, FEI Hongrong, ZHAO Ying. Extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 537-542. doi: 10.3969/j.issn.1006-0111.2019.06.013
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Extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa

doi: 10.3969/j.issn.1006-0111.2019.06.013

  • Shandong First Medical University&Shandong Academy of Medical Sciences, Taian 271016, China

  • Received Date: 2018-09-19
  • Rev Recd Date: 2019-11-04
  • Objective To screen out the optimal conditions for extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa. Methods Single factor and L9(34) orthogonal experiment was designed with the extraction ratio and extract quantity of total flavonoids as indexes,using matching of herb pair,ethanol concentration,solid-liquid ratio,extraction time as inspected elements.The technical process for purification of total flavonoids with the static and dynamic adsorption experiments was screened by adsorption and desorption ratio as targets. Results The optimal process conditions were:ethanol concentration 70%,the matching of herb pair of 4:1,the solid-liquid ratio of 1:25,extraction for 3 times and 2h for each time.The parameters of the purification process were as follows:concentration of sample 63.14 mg/ml and pH value 4.0,which used in the chromatographic column of polyamide resin at a flow velocity of 1.5 ml/min.The chromatographic column was eluted with purified water of 3.1BV,and then eluted with 65% ethanol concentration of 9.3BV at a flow velocity of 3ml/min.The purity of the total flavonoids was 40.39% in the fine product,and the transfer rate was 81.57%. Conclusion The optimal process conditions were reasonable and feasible.The purification technique of polyamide resin was uncomplicated and had a good effect.
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    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 20.2 %其他: 20.2 %China: 1.4 %China: 1.4 %India: 0.4 %India: 0.4 %上海: 2.8 %上海: 2.8 %东莞: 0.4 %东莞: 0.4 %临汾: 0.4 %临汾: 0.4 %乌鲁木齐: 0.2 %乌鲁木齐: 0.2 %信阳: 0.2 %信阳: 0.2 %北京: 11.9 %北京: 11.9 %南京: 0.2 %南京: 0.2 %南宁: 0.2 %南宁: 0.2 %南昌: 0.5 %南昌: 0.5 %南通: 0.2 %南通: 0.2 %台湾省: 0.2 %台湾省: 0.2 %合肥: 0.2 %合肥: 0.2 %咸阳: 0.5 %咸阳: 0.5 %唐山: 1.2 %唐山: 1.2 %大连: 0.9 %大连: 0.9 %天津: 0.4 %天津: 0.4 %孝感: 0.2 %孝感: 0.2 %安顺: 0.2 %安顺: 0.2 %广州: 0.9 %广州: 0.9 %张家口: 0.7 %张家口: 0.7 %惠州: 0.2 %惠州: 0.2 %成都: 0.7 %成都: 0.7 %无锡: 0.2 %无锡: 0.2 %昆明: 0.2 %昆明: 0.2 %杭州: 0.4 %杭州: 0.4 %梧州: 0.2 %梧州: 0.2 %武汉: 0.5 %武汉: 0.5 %洛阳: 0.2 %洛阳: 0.2 %济南: 0.2 %济南: 0.2 %深圳: 0.7 %深圳: 0.7 %漳州: 0.2 %漳州: 0.2 %濮阳: 0.2 %濮阳: 0.2 %盐城: 0.4 %盐城: 0.4 %石家庄: 0.5 %石家庄: 0.5 %聊城: 0.2 %聊城: 0.2 %芒廷维尤: 26.2 %芒廷维尤: 26.2 %芝加哥: 0.2 %芝加哥: 0.2 %苏州: 0.5 %苏州: 0.5 %菏泽: 0.2 %菏泽: 0.2 %西宁: 20.5 %西宁: 20.5 %西安: 0.2 %西安: 0.2 %赤峰: 0.4 %赤峰: 0.4 %运城: 0.9 %运城: 0.9 %郑州: 0.2 %郑州: 0.2 %长沙: 0.4 %长沙: 0.4 %长治: 0.5 %长治: 0.5 %黄冈: 0.9 %黄冈: 0.9 %黄冈市麻城: 0.2 %黄冈市麻城: 0.2 %其他ChinaIndia上海东莞临汾乌鲁木齐信阳北京南京南宁南昌南通台湾省合肥咸阳唐山大连天津孝感安顺广州张家口惠州成都无锡昆明杭州梧州武汉洛阳济南深圳漳州濮阳盐城石家庄聊城芒廷维尤芝加哥苏州菏泽西宁西安赤峰运城郑州长沙长治黄冈黄冈市麻城Highcharts.com
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Extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa

doi: 10.3969/j.issn.1006-0111.2019.06.013
  • Shandong First Medical University&Shandong Academy of Medical Sciences, Taian 271016, China
  • Received Date: 2018-09-19
  • Rev Recd Date: 2019-11-04

Abstract: Objective To screen out the optimal conditions for extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa. Methods Single factor and L9(34) orthogonal experiment was designed with the extraction ratio and extract quantity of total flavonoids as indexes,using matching of herb pair,ethanol concentration,solid-liquid ratio,extraction time as inspected elements.The technical process for purification of total flavonoids with the static and dynamic adsorption experiments was screened by adsorption and desorption ratio as targets. Results The optimal process conditions were:ethanol concentration 70%,the matching of herb pair of 4:1,the solid-liquid ratio of 1:25,extraction for 3 times and 2h for each time.The parameters of the purification process were as follows:concentration of sample 63.14 mg/ml and pH value 4.0,which used in the chromatographic column of polyamide resin at a flow velocity of 1.5 ml/min.The chromatographic column was eluted with purified water of 3.1BV,and then eluted with 65% ethanol concentration of 9.3BV at a flow velocity of 3ml/min.The purity of the total flavonoids was 40.39% in the fine product,and the transfer rate was 81.57%. Conclusion The optimal process conditions were reasonable and feasible.The purification technique of polyamide resin was uncomplicated and had a good effect.

GAO Yanxia, SU Juan, JIN Huizi. Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 249-251,254. doi: 10.3969/j.issn.1006-0111.2016.03.014
Citation: WANG Guiling, FANG Jianqiang, FEI Hongrong, ZHAO Ying. Extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 537-542. doi: 10.3969/j.issn.1006-0111.2019.06.013
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