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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2019  >  37(6): 498-502

DING Huamin, QIN Chunxia, MA Kaiyuan, SUN Lili, ZHANG Yuefan, LI Tiejun. Protective effect of MLIF on traumatic brain injury in rats[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 498-502. doi: 10.3969/j.issn.1006-0111.2019.06.005
Citation: DING Huamin, QIN Chunxia, MA Kaiyuan, SUN Lili, ZHANG Yuefan, LI Tiejun. Protective effect of MLIF on traumatic brain injury in rats[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 498-502. doi: 10.3969/j.issn.1006-0111.2019.06.005
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Protective effect of MLIF on traumatic brain injury in rats

doi: 10.3969/j.issn.1006-0111.2019.06.005
  • 1.

    Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, China

  • 2.

    School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China

  • 3.

    Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, China;School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China

  • Received Date: 2019-07-23
  • Rev Recd Date: 2019-10-08
  • Objective To study the protective effect and explore the mechanism of monocyte locomotion inhibitor factor(MLIF) on traumatic brain injury (TBI) in rats. Methods The SD rats were randomly divided into five groups:sham operation group,model group and three MLIF groups (0.33 mg/kg,1 mg/kg,3 mg/kg).Rats were made model of brain injury by fluid percussion injury.The tail vein was administered within 30 min after TBI,once every 24 hours,and rats were administered for 7 days.Brain water content,serum levels of SOD and MDA,Hematoxylin-eosin staining and Nissl staining were used to detect the rats brain tissues. Results After rats traumatic brain injury for 24h,the brain water content in rats in three MLIF groups(0.33 mg/kg,1 mg/kg and 3 mg/kg)was significantly lower than that in model group(P<0.01),The effect of MLIF (1 mg/kg) group was the best.Compared to rats traumatic brain injury for 1d and 3d,brain water content in MLIF group (1 mg/kg) was significantly lower than that in model group (P<0.01).SOD content in serum in MLIF (1 mg/kg) group was significantly increased (P <0.01) and MDA content in serum in MLIF (1 mg/kg) group was significantly decreased (P<0.05).HE staining and Nissl staining showed that the pathological changes of brain tissue in model group were obvious,and which in MLIF group were improved in brain tissue. Conclusion MLIF could inhibit oxidative stress and edema reaction of brain injury,which had protective effect on traumatic brain injury.
  • [1] LOANE D J,FADEN A I.Neuroprotection for traumatic brain injury:translational challenges and emerging therapeutic strategies[J].Trends Pharmacol Sci,2010,31(12):596-604.
    [2] RICO G,LEANDRO E,ROJAS S,et al.The effect of the monocyte locomotion inhibitory factor (MLIF) produced by Entamoeba histolytica upon nitric oxide production by human leukocytes[J].Arch Med Res,2000,31(4 Suppl):S90-S91.
    [3] ZHANG Y F,CHEN J,LI F,et al.A pentapeptide monocyte locomotion inhibitory factor protects brain ischemia injury by targeting the eEF1A1/endothelial nitric oxide synthase pathway[J].Stroke,2012,43(10):2764-2773.
    [4] 程浩,芮耀诚,章越凡,等.生物活性肽-单核细胞迁移抑制因子研究进展[J].药学实践杂志,2015,33(1):17-19,27.
    [5] ZHU Q Z,ZHANG Y F,LIU Y L,et al.MLIF alleviates SH-SY5Y neuroblastoma injury induced by oxygen-glucose deprivation by targeting eukaryotic translation elongation factor 1A2[J].PLoS One,2016,11(2):e0149965.
    [6] MCINTOSH T K,VINK R,NOBLE L,et al.Traumatic brain injury in the rat:characterization of a lateral fluid-percussion model[J].Neuroscience,1989,28(1):233-244.
    [7] MARKLUND N,HILLERED L.Animal modelling of traumatic brain injury in preclinical drug development:where do we go from here?[J].Br J Pharmacol,2011,164(4):1207-1229.
    [8] FUKUDA A M,BADAUT J.Aquaporin 4:a player in cerebral edema and neuroinflammation[J].J Neuroinflammation,2012,9:279.
    [9] MARMAROU A.Pathophysiology of traumatic brain edema:current concepts[J].Acta Neurochir Suppl,2003,86:7-10.
    [10] FEICKERT H J,DROMMER S,HEYER R.Severe head injury in children:impact of risk factors on outcome[J].J Trauma,1999,47(1):33-38.
    [11] DONKIN J J,VINK R.Mechanisms of cerebral edema in traumatic brain injury:therapeutic developments[J].Curr Opin Neurol,2010,23(3):293-299.
    [12] HALL E D,VAISHNAV R A,MUSTAFA A G.Antioxidant therapies for traumatic brain injury[J].Neurotherapeutics,2010,7(1):51-61.
    [13] JI X T,LIU W B,XIE K L,et al.Beneficial effects of hydrogen gas in a rat model of traumatic brain injury via reducing oxidative stress[J].Brain Res,2010,1354:196-205.
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Protective effect of MLIF on traumatic brain injury in rats

doi: 10.3969/j.issn.1006-0111.2019.06.005
  • 1. Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, China
  • 2. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
  • 3. Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, China;School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
  • Received Date: 2019-07-23
  • Rev Recd Date: 2019-10-08

Abstract: Objective To study the protective effect and explore the mechanism of monocyte locomotion inhibitor factor(MLIF) on traumatic brain injury (TBI) in rats. Methods The SD rats were randomly divided into five groups:sham operation group,model group and three MLIF groups (0.33 mg/kg,1 mg/kg,3 mg/kg).Rats were made model of brain injury by fluid percussion injury.The tail vein was administered within 30 min after TBI,once every 24 hours,and rats were administered for 7 days.Brain water content,serum levels of SOD and MDA,Hematoxylin-eosin staining and Nissl staining were used to detect the rats brain tissues. Results After rats traumatic brain injury for 24h,the brain water content in rats in three MLIF groups(0.33 mg/kg,1 mg/kg and 3 mg/kg)was significantly lower than that in model group(P<0.01),The effect of MLIF (1 mg/kg) group was the best.Compared to rats traumatic brain injury for 1d and 3d,brain water content in MLIF group (1 mg/kg) was significantly lower than that in model group (P<0.01).SOD content in serum in MLIF (1 mg/kg) group was significantly increased (P <0.01) and MDA content in serum in MLIF (1 mg/kg) group was significantly decreased (P<0.05).HE staining and Nissl staining showed that the pathological changes of brain tissue in model group were obvious,and which in MLIF group were improved in brain tissue. Conclusion MLIF could inhibit oxidative stress and edema reaction of brain injury,which had protective effect on traumatic brain injury.

DING Huamin, QIN Chunxia, MA Kaiyuan, SUN Lili, ZHANG Yuefan, LI Tiejun. Protective effect of MLIF on traumatic brain injury in rats[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 498-502. doi: 10.3969/j.issn.1006-0111.2019.06.005
Citation: DING Huamin, QIN Chunxia, MA Kaiyuan, SUN Lili, ZHANG Yuefan, LI Tiejun. Protective effect of MLIF on traumatic brain injury in rats[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 498-502. doi: 10.3969/j.issn.1006-0111.2019.06.005
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