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Volume 38 Issue 1
Mar.  2020
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2020  >  38(1): 57-62

MA Minghua, WANG Xiaohe, DAI Yuanyuan, GU Xiaoyan, WU Tiejun, NIAN Hua. Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 57-62. doi: 10.3969/j.issn.1006-0111.201812070
Citation: MA Minghua, WANG Xiaohe, DAI Yuanyuan, GU Xiaoyan, WU Tiejun, NIAN Hua. Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 57-62. doi: 10.3969/j.issn.1006-0111.201812070
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Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice

doi: 10.3969/j.issn.1006-0111.201812070
  • 1.

    Pharmaceutical Center of Yangpu District Central Hospital, Shanghai 200090, China

  • 2.

    Pharmaceutical Center of Yueyang Hospital, Shanghai University of TCM, Shanghai 200437, China

  • Received Date: 2018-12-24
  • Rev Recd Date: 2019-09-22
  • Objective To investigate the therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice. Methods 30 mice with C57BL/6 mouse Lewis lung cancer xenograft model were randomly divided into three groups:model control group, Xiakucao Xiaoliu mixture group (M group), cisplatin group (DDP group). M group and DDP group were administered continuously for 14 days. Through the general observation of Lewis lung cancer mice, tumor size was determined, HE staining method was used to determine the histopathological changes of tumors, and the expression of CyclinD1 and P16 in tumor tissues was determined by immunohistochemistry. Results The tumor weight of the model control group was the heaviest, and the difference was statistically significant compared with other groups. (P<0.05). Survival state and quality of life of mice had been improved to some extent in M group. The results of tumor growth curve and HE staining in each group of mice showed that the growth of tumor cells had been inhibited and normal cells had been protected. The positive expression of CyclinD1 was significantly decreased in M group and DDP group (P<0.01), but the effect of M group on the improvement of P16 positive expression was not significant. Conclusion Xiakucao Xiaoliu mixture had a good effect on inhibiting lung tumor growth.
  • [1] TORRE L A, BRAY F, SIEGEL R L, et al. Global cancer statistics, 2012[J]. CA: Cancer J Clin,2015,65(2):87-108
    [2] 赵东杰. 中西医结合疗法对老年晚期非小细胞肺癌临床疗效的影响[J]. 中外医疗, 2017, 36(9):167-169
    [3] 王丽丽, 石磊, 钱祥, 等. 化疗联合清肺合剂改善非小细胞肺癌患者免疫功能的临床研究[J]. 中华全科医学, 2017, 15(7):1166-1168
    [4] 尚兴平, 齐敏. 益气养阴法联合化疗治疗晚期非小细胞肺癌的疗效观察[J]. 中国继续医学教育, 2017, 9(21):203-205
    [5] 柯军. 迷迭香酸抑制肺癌A549细胞的增殖及侵袭的实验研究[J]. 甘肃医药, 2015, 34(4):255-258
    [6] 贺天临, 谢国群, 陈洁, 等. 夏枯草消瘤合剂联合化疗治疗中晚期非小细胞肺癌30例[J]. 上海中医药大学学报, 2015, 29(5):29-32
    [7] 马雪曼, 于明薇, 张甘霖, 等. 小鼠Lewis肺癌不同部位皮下移植瘤模型的比较[J]. 中国实验动物学报, 2017, 25(4):386-390
    [8] 靳福鹏, 张梅, 李平, 等. 益气养阴解毒方对小鼠Lewis肺癌生长抑制作用的实验研究[J]. 临床肺科杂志, 2011, 16(1):72-74
    [9] 钱超尘, 温长路,赵怀舟,等(校). 金陵本《本草纲目》新校正[M]. 上海: 上海科学技术出版社, 2008.
    [10] 严东, 谢文剑, 李春, 等. 夏枯草化学成分及其体外抗肿瘤活性研究[J]. 中国实验方剂学杂志, 2016, 22(11):49-54
    [11] 张朝晖, 徐正顺, 焦龙华, 等. CyclinD1, P16, Rb蛋白在非小细胞肺癌组织中的表达[J]. 第四军医大学学报, 2003, 24(14):1271-1274
    [12] 王前, 邓晶, 蒋永新. CyclinD1的研究进展[J]. 现代肿瘤医学, 2009, 17(2):350-353
    [13] CHOI Y J, LI X Y, HYDBRING P, et al. The requirement for Cyclin D function in tumor maintenance[J]. Cancer Cell,2012,22(4):438-451
    [14] MASAMHA C P, BENBROOK D M. Cyclin D1 degradation is sufficient to induce G1 cell cycle arrest despite constitutive expression of Cyclin E2 in ovarian cancer cells[J]. Cancer Res,2009,69(16):6565-6572
    [15] OGINO S, NOSHO K, IRAHARA N, et al. A cohort study of Cyclin D1 expression and prognosis in 602 colon cancer cases[J]. Clin Cancer Res,2009,15(13):4431-4438
    [16] ISHIKAWA T, FURIHATA M, OHTSUKI Y, et al. Cyclin D1 overexpression related to retinoblastoma protein expression as a prognostic marker in human oesophageal squamous cell carcinoma[J]. Br J Cancer,1998,77(1):92-97
    [17] LI H Y, XIAO W W, MA J W, et al. Dual high expression of STAT3 and CyclinD1 is associated with poor prognosis after curative resection of esophageal squamous cell carcinoma[J]. Int J Clin Exp Pathol,2014,7(11):7989-7998
    [18] 夏靖华, 李维妙, 王雪娇, 等. CyclinD1与CyclinD3在非小细胞肺癌中的表达及意义[J]. 肿瘤学杂志, 2016, 22(8):615-621
    [19] 梁瑞韵, 廖增顺, 江山平, 等. CyclinD1和VEGF在非小细胞肺癌组织中的表达及其与预后的关系[J]. 癌症, 2003, 22(1):86-90
    [20] 付冉, 王艳林, 黄利鸣. p16表达异常与肿瘤关系的研究进展[J]. 生命的化学, 2017, 37(4):585-589
    [21] 冯蓓, 冯艳, 杨小平, 等. P16在肺癌方面的研究进展[J]. 延安大学学报(医学科学版), 2015, 13(1):70-73
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Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice

doi: 10.3969/j.issn.1006-0111.201812070
  • 1. Pharmaceutical Center of Yangpu District Central Hospital, Shanghai 200090, China
  • 2. Pharmaceutical Center of Yueyang Hospital, Shanghai University of TCM, Shanghai 200437, China
  • Received Date: 2018-12-24
  • Rev Recd Date: 2019-09-22

Abstract: Objective To investigate the therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice. Methods 30 mice with C57BL/6 mouse Lewis lung cancer xenograft model were randomly divided into three groups:model control group, Xiakucao Xiaoliu mixture group (M group), cisplatin group (DDP group). M group and DDP group were administered continuously for 14 days. Through the general observation of Lewis lung cancer mice, tumor size was determined, HE staining method was used to determine the histopathological changes of tumors, and the expression of CyclinD1 and P16 in tumor tissues was determined by immunohistochemistry. Results The tumor weight of the model control group was the heaviest, and the difference was statistically significant compared with other groups. (P<0.05). Survival state and quality of life of mice had been improved to some extent in M group. The results of tumor growth curve and HE staining in each group of mice showed that the growth of tumor cells had been inhibited and normal cells had been protected. The positive expression of CyclinD1 was significantly decreased in M group and DDP group (P<0.01), but the effect of M group on the improvement of P16 positive expression was not significant. Conclusion Xiakucao Xiaoliu mixture had a good effect on inhibiting lung tumor growth.

MA Minghua, WANG Xiaohe, DAI Yuanyuan, GU Xiaoyan, WU Tiejun, NIAN Hua. Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 57-62. doi: 10.3969/j.issn.1006-0111.201812070
Citation: MA Minghua, WANG Xiaohe, DAI Yuanyuan, GU Xiaoyan, WU Tiejun, NIAN Hua. Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 57-62. doi: 10.3969/j.issn.1006-0111.201812070
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