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GAO Yanxia, SU Juan, JIN Huizi. Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 249-251,254. doi: 10.3969/j.issn.1006-0111.2016.03.014
Citation:
ZHANG Zhen, FENG Jiayi, GUO Hui, YAO Yuan, WEN Jun, ZHOU Tingting. Simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(4): 347-350. doi: 10.3969/j.issn.1006-0111.2018.04.013
Simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro by HPLC
School of Pharmacy & Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Second Military Medical University Shanghai 200433, China
2.
School of Pharmacy & Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Second Military Medical University Shanghai 200433, China;School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
Received Date: 2017-10-02
Rev Recd Date:
2018-03-26
Abstract
Objective To establish an HPLC method for simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro, and study the metabolism of daidzin by rat intestinal bacteria. Methods The incubation of daidzin was carried out at 37℃ with rat intestinal bacteria test solution. The samples were precipitated by methanol followed by a separation with HPLC method. The conditions were as follows:the chromatographic column was Ultimate C18; the mobile phase was 0.2% acetic acid and methanol (gradient elution); the detection wavelength was 260nm, and the column temperature was 25℃. Results Daidzin and daidzein showed good linearity with the range of 98.0~490μg/ml and 57.6~288μg/ml, respectively. The accuracy of daidzin and daidzein was ranged from 96.6%~114.0% and the precision of the two analytes was within 9.9%. Daidzin was transformed to daidzein in the rat intestinal bacteria test solution, and its elimination half-life was 37.6 min. Conclusion The established HPLC method was suitable for the determination of daidzin and its metabolite. Daidzin could be metabolized by rat intestinal bacteria in vitro,and the main metabolic pathway was transforming daidzin into corresponding aglycone through breaking the glucoside bond
SILBERBERG M,MORAND C,MATHEVON T,et al. The bioavailability of polyphenols is highly governed by the capacity of the intestine and of the liver to secrete conjugated metabolites[J]. Eur J Nutr,2006,45(2):88-96.
VITALE DC,PIAZZA C,MELILLI B,et al. Isoflavones:estrogenic activity,biological effect and bioavailability[J]. Eur J Drug Metab Ph,2013,38(1):15-25.
[6]
SETCHELL KD,BROWN NM,ZIMMER-NECHEMIAS L,et al. Evidence for lack of absorption of soy isoflavone glycosides in humans,supporting the crucial role of intestinal metabolism for bioavailability[J]. Am J Clin Nutr,2002,76(2):447-453.
[7]
PISKULA MK,YAMAKOSHI J,IWAI Y. Daidzein and genistein but not their glucosides are absorbed from the rat stomach[J]. Febs Lett,1999,447(2-3):287-291.
[8]
CHANDRASEKHARAN S,AGLIN A. Pharmacokinetics of dietary isoflavones[J]. J Steroids Hormon Sci 2013,12(1):4.
1. School of Pharmacy & Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Second Military Medical University Shanghai 200433, China
2. School of Pharmacy & Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Second Military Medical University Shanghai 200433, China;School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
Abstract: Objective To establish an HPLC method for simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro, and study the metabolism of daidzin by rat intestinal bacteria. Methods The incubation of daidzin was carried out at 37℃ with rat intestinal bacteria test solution. The samples were precipitated by methanol followed by a separation with HPLC method. The conditions were as follows:the chromatographic column was Ultimate C18; the mobile phase was 0.2% acetic acid and methanol (gradient elution); the detection wavelength was 260nm, and the column temperature was 25℃. Results Daidzin and daidzein showed good linearity with the range of 98.0~490μg/ml and 57.6~288μg/ml, respectively. The accuracy of daidzin and daidzein was ranged from 96.6%~114.0% and the precision of the two analytes was within 9.9%. Daidzin was transformed to daidzein in the rat intestinal bacteria test solution, and its elimination half-life was 37.6 min. Conclusion The established HPLC method was suitable for the determination of daidzin and its metabolite. Daidzin could be metabolized by rat intestinal bacteria in vitro,and the main metabolic pathway was transforming daidzin into corresponding aglycone through breaking the glucoside bond
GAO Yanxia, SU Juan, JIN Huizi. Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 249-251,254. doi: 10.3969/j.issn.1006-0111.2016.03.014
Citation:
ZHANG Zhen, FENG Jiayi, GUO Hui, YAO Yuan, WEN Jun, ZHOU Tingting. Simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(4): 347-350. doi: 10.3969/j.issn.1006-0111.2018.04.013
SILBERBERG M,MORAND C,MATHEVON T,et al. The bioavailability of polyphenols is highly governed by the capacity of the intestine and of the liver to secrete conjugated metabolites[J]. Eur J Nutr,2006,45(2):88-96.
VITALE DC,PIAZZA C,MELILLI B,et al. Isoflavones:estrogenic activity,biological effect and bioavailability[J]. Eur J Drug Metab Ph,2013,38(1):15-25.
[6]
SETCHELL KD,BROWN NM,ZIMMER-NECHEMIAS L,et al. Evidence for lack of absorption of soy isoflavone glycosides in humans,supporting the crucial role of intestinal metabolism for bioavailability[J]. Am J Clin Nutr,2002,76(2):447-453.
[7]
PISKULA MK,YAMAKOSHI J,IWAI Y. Daidzein and genistein but not their glucosides are absorbed from the rat stomach[J]. Febs Lett,1999,447(2-3):287-291.
[8]
CHANDRASEKHARAN S,AGLIN A. Pharmacokinetics of dietary isoflavones[J]. J Steroids Hormon Sci 2013,12(1):4.
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GAO Yanxia, SU Juan, JIN Huizi. Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 249-251,254. doi: 10.3969/j.issn.1006-0111.2016.03.014
GAO Yanxia, SU Juan, JIN Huizi. Study on HPLC fingerprint of Ginseng Radix et Rhizoma Rubra[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 249-251,254. doi: 10.3969/j.issn.1006-0111.2016.03.014
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