MAO Ling, WEI Guobing, YE Ji, ZHOU Bailun, LIN Yinan. Simultaneous determination of oleanolic acid and ursolic acid in Hawthorn extract by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(6): 537-539,555. doi: 10.3969/j.issn.1006-0111.2016.06.014
| Citation:
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MAO Ling, WEI Guobing, YE Ji, ZHOU Bailun, LIN Yinan. Simultaneous determination of oleanolic acid and ursolic acid in Hawthorn extract by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(6): 537-539,555. doi: 10.3969/j.issn.1006-0111.2016.06.014
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Simultaneous determination of oleanolic acid and ursolic acid in Hawthorn extract by HPLC
- 1.
School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China;School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- 2.
School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China
- 3.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- 4.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China;School of Traditional Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China
- 5.
School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China
- Received Date: 2016-04-05
- Rev Recd Date:
2016-06-01
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Abstract
Objective To establish a HPLC method for simultaneous determination of oleanolic acid and ursolic acid and provide the basis for quality control of Hawthorn extract. Methods Chromatographic separation was performed on Agilent Zorbax SB C18 column(250 mm×4.6 mm, 5 μm) with mobile phase of methanol (A)-0.06 mol/L ammonium acetate solution (B) (85:15, V/V) under isocratic elution for 30 min. The flow rate was set at 0.8 ml/min and the detection was set at the wavelength of 210 nm. Results Oleanolic acid and ursolic acid showed good linearity (r> 0.999 5) in the ranges of 0.496-2.480 g and 0.498-9.960 g, respectively. Repeatability, precision, recovery and stability were conform to the method validation requirements of China Pharmacopoeia. Conclusion The method could provide the basis for the quality control of Hawthorn extract and its preparation.
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