Study on quality standards of hospital preparation Ganmao granules

JIN Weihua; YU Botao; CHEN Hua; ZHANG Ming; FAN Kaihua; PU Zhiqiang; SONG Zonghui

doi:10.3969/j.issn.1006-0111.2016.06.013

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To establish a quality standard for Ganmao granules of hospital preparations. Methods Radix Scutellariae, cortex phellodendri and radix bupleuri were identified by thin layer chromatography (TLC) qualitatively. High performance liquid chromatography (HPLC) was used for the content of baicalin. The determination was performed on Agilent HC-C₁₈ column (250 mm×4.6 mm,5 μm) at 30℃ with mobile phase composed of methanol -0.2% phosphoric acid (43:57) at the flow rate of 1.0 ml/min. The detection wavelength was set at 280 nm. Results In TLC chromatograms, the spectra of different test products had spots of the same color at corresponding sites, with no interference from negative control. A good linearity range of baicalin was 1.81~72.40 μg/ml (r=0.999 9). The average recovery rate was 98.55% (RSD=1.91%, n=9). Conclusion The quality standard was established and the method of identification has good reproducibility. The method of determination of baicalin content improved the controllability of formulated quality of Ganmao granules.

Study on quality standards of hospital preparation Ganmao granules

Department of Pharmacy, General Hospital of Chengdu Military Command, Chengdu 610083, China

Received Date: 2015-07-30

Rev Recd Date: 2016-04-28

Key words:

Ganmao granules /
baicalin /
TLC /
HPLC

Abstract: Objective To establish a quality standard for Ganmao granules of hospital preparations. Methods Radix Scutellariae, cortex phellodendri and radix bupleuri were identified by thin layer chromatography (TLC) qualitatively. High performance liquid chromatography (HPLC) was used for the content of baicalin. The determination was performed on Agilent HC-C₁₈ column (250 mm×4.6 mm,5 μm) at 30℃ with mobile phase composed of methanol -0.2% phosphoric acid (43:57) at the flow rate of 1.0 ml/min. The detection wavelength was set at 280 nm. Results In TLC chromatograms, the spectra of different test products had spots of the same color at corresponding sites, with no interference from negative control. A good linearity range of baicalin was 1.81~72.40 μg/ml (r=0.999 9). The average recovery rate was 98.55% (RSD=1.91%, n=9). Conclusion The quality standard was established and the method of identification has good reproducibility. The method of determination of baicalin content improved the controllability of formulated quality of Ganmao granules.

HTML

Reference (9)

[1]	国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药科技出版社,2010附录Ⅵ B:34.
[2]	江涛,雷健,邢茂,等.口炎清胶囊的质量控制和稳定性试验[J].中国药业,2014,23(11):17-20.
[3]	黄晓巧,李养学,胥爱丽,等.复方红黄洗剂质量标准研究[J].江西中医药,2014,45(8):73-75.
[4]	何小英,黄艳萍. 胃肠乐片的质量标准研究[J].今日药学,2014,24(10):726-728.
[5]	刘香玉,田民强,冯丽莉,等.复方解郁颗粒的质量标准研究[J].中国药师,2014,17(5):763-766.
[6]	林海霞,王书林,王砚,等.HPLC测定竹叶柴胡中黄酮类成分的含量[J].中国实验方剂学杂志,2012,18(15):76-79.
[7]	刘红,刘辉,刘彬果. 高效液相色谱法测定急麻颗粒中黄芩苷的含量[J].天津药学,2014,26(6):11-12.
[8]	乡世健,翁立冬,黄德浩,等. HPLC测定小儿连番止泻颗粒中黄芩苷的含量[J].今日药学,2014,24(11):781-783.
[9]	尹亚梅. HPLC法同时测定芩翘口服液中黄芩苷、连翘苷的含量[J].中国药师,2014,17(11):1973-1975.

Message Board

Study on quality standards of hospital preparation Ganmao granules

doi: 10.3969/j.issn.1006-0111.2016.06.013

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Related

Proportional views