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SHEN Liru, LI Ruihong, WANG Tailiang. Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
Citation:
DONG Wei, YAN Jianwei, TAN Qiaoling, YE Sen. Effect of the curcumin on expression of IL-2 and IL-6 of hippocampus in pentylenetetrazol-induced epilepsy in rats[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 354-356. doi: 10.3969/j.issn.1006-0111.2016.04.018
Effect of the curcumin on expression of IL-2 and IL-6 of hippocampus in pentylenetetrazol-induced epilepsy in rats
Objective To investigate the mechanism of anti-epileptic effect of the curcumin. Methods The SD rats were injected intraperitoneally with pentylenetetrazol kindling 25.0 mg/kg to induce a rat epilepsy model. All of the treatments were performed once a day continuously for 28 days. The rats in blank group and model group received 5 ml of normal saline. The rats in the high and low curcumin group were given 200 mg/kg and 100 mg/kg of curcumin once a day, respectively. The rats in the sodium valproate (VPA) group were given 400 mg/kg of VPA once a day by gavage. After treatment, the seizures level was recorded by using the Racine's six point grading scale, and the expression of IL-2 and IL-6 of hippocampus were detected by the enzyme linked immunoassay (ELISA). Results The seizures level was reduced by curcumin in epileptic rats. The expressions of IL-2 and IL-6 of the model group were significantly higher than those of the blank group (P<0.05), while those rats of the anti-epileptic groups, including high dose group and low dose group, were lower than those rats of the model group (P<0.05). When compared with the curcumin low dose group, the expression of IL-2 and IL-6 of curcumin high dose group is lower (P<0.05). There was no significant difference between the high dose curcumin group and VPA group (P>0.05). Conclusion The curcumin can reduce the seizure level in rats, it shows some anti-epileptic effets and dose-dependently, which may be through down-regulating the expression of IL-2 and IL-6 in hippocampus.
RavizzaT, Rizzi M, Perego C, et a1. Inflammatory response and glia activation in developing rat hippoeampus after status epilepticus[J]. Epilepsia,2005,46(S5):113-117.
[5]
Lehtimki KA, Kernen T, Huhtala H, et al. Regulation of IL-6 system in cerebrospinal fluid and serum compartments by seizures:the effect of seizure type and duration[J]. J Neuroimmunol.2004,152(1-2):121-125.
[6]
Racine RJ. Modification of seizure activity by electrical stimulation Ⅱ. motor seizure[J]. Electroencephalogr Clin Neurophysiol,1972,32(3):281-294.
Shin HJ, Lee JY, Son E, et al. Curcumin attenuates the kainic acid-induced hippocampal cell death in the mice[J]. Neurosci Lett,2007,416(1):49-54.
[9]
Agarwal NB, Jain S, Agarwal NK, et al.Modulation of pentylenetetrazole-induced kindling andoxidative stress by curcumin in mice[J]. Phytomedicine,2011, 18(8-9):756-759.
[10]
Mehla J, Reeta KH, Gupta P, et al. Protective effects of curcumin against seizares and congnitive impairment in a pentylenetetrazole-kindled epileptic rat model[J].Life Sci, 2010, 87(19-22):596-603.
Abstract: Objective To investigate the mechanism of anti-epileptic effect of the curcumin. Methods The SD rats were injected intraperitoneally with pentylenetetrazol kindling 25.0 mg/kg to induce a rat epilepsy model. All of the treatments were performed once a day continuously for 28 days. The rats in blank group and model group received 5 ml of normal saline. The rats in the high and low curcumin group were given 200 mg/kg and 100 mg/kg of curcumin once a day, respectively. The rats in the sodium valproate (VPA) group were given 400 mg/kg of VPA once a day by gavage. After treatment, the seizures level was recorded by using the Racine's six point grading scale, and the expression of IL-2 and IL-6 of hippocampus were detected by the enzyme linked immunoassay (ELISA). Results The seizures level was reduced by curcumin in epileptic rats. The expressions of IL-2 and IL-6 of the model group were significantly higher than those of the blank group (P<0.05), while those rats of the anti-epileptic groups, including high dose group and low dose group, were lower than those rats of the model group (P<0.05). When compared with the curcumin low dose group, the expression of IL-2 and IL-6 of curcumin high dose group is lower (P<0.05). There was no significant difference between the high dose curcumin group and VPA group (P>0.05). Conclusion The curcumin can reduce the seizure level in rats, it shows some anti-epileptic effets and dose-dependently, which may be through down-regulating the expression of IL-2 and IL-6 in hippocampus.
SHEN Liru, LI Ruihong, WANG Tailiang. Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
Citation:
DONG Wei, YAN Jianwei, TAN Qiaoling, YE Sen. Effect of the curcumin on expression of IL-2 and IL-6 of hippocampus in pentylenetetrazol-induced epilepsy in rats[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 354-356. doi: 10.3969/j.issn.1006-0111.2016.04.018
RavizzaT, Rizzi M, Perego C, et a1. Inflammatory response and glia activation in developing rat hippoeampus after status epilepticus[J]. Epilepsia,2005,46(S5):113-117.
[5]
Lehtimki KA, Kernen T, Huhtala H, et al. Regulation of IL-6 system in cerebrospinal fluid and serum compartments by seizures:the effect of seizure type and duration[J]. J Neuroimmunol.2004,152(1-2):121-125.
[6]
Racine RJ. Modification of seizure activity by electrical stimulation Ⅱ. motor seizure[J]. Electroencephalogr Clin Neurophysiol,1972,32(3):281-294.
Shin HJ, Lee JY, Son E, et al. Curcumin attenuates the kainic acid-induced hippocampal cell death in the mice[J]. Neurosci Lett,2007,416(1):49-54.
[9]
Agarwal NB, Jain S, Agarwal NK, et al.Modulation of pentylenetetrazole-induced kindling andoxidative stress by curcumin in mice[J]. Phytomedicine,2011, 18(8-9):756-759.
[10]
Mehla J, Reeta KH, Gupta P, et al. Protective effects of curcumin against seizares and congnitive impairment in a pentylenetetrazole-kindled epileptic rat model[J].Life Sci, 2010, 87(19-22):596-603.
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SHEN Liru, LI Ruihong, WANG Tailiang. Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
SHEN Liru, LI Ruihong, WANG Tailiang. Simultaneous determination of peimine and peiminine in Xiaoer Baotaikang granules by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 351-353. doi: 10.3969/j.issn.1006-0111.2016.04.017
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