Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!
ZHU Hengmei, LU Guihua, FANG Shuheng, XU Lili. Pain Management with fentanyl transdermal and morphine subcutaneous injection for the patients with advanced liver cancer pain after intervention therapy[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(1): 75-77. doi: 10.3969/j.issn.1006-0111.2017.01.019
Citation:
REN Xinxin, WANG Xianyou, LU Wenquan, Piao Shujuan, GAO Shouhong. Study on quality control of Xinnaoling granules[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 338-342. doi: 10.3969/j.issn.1006-0111.2016.04.014
Department of Pharmacy, Changzheng Hospital Affiliated to Second Military Medical University, Shanghai 200433, China;School of Pharmacy, Henan University, Kaifeng 475000, China
2.
School of Pharmacy, Henan University, Kaifeng 475000, China
3.
Department of Pharmacy, Changzheng Hospital Affiliated to Second Military Medical University, Shanghai 200433, China
Received Date: 2015-09-28
Rev Recd Date:
2015-12-01
Abstract
Objective To establish quality control of Xinnaoling granules. Methods Thin layer chromatography (TLC) methods were used to identify Rhizoma Corydalis, Radix Angelicae dahuricae, Cortex Magnoliae officinalis, Radix Glycyrrhizae, Rhizoma Ligusticum, Ligusticum wallichii, Schisandra Chinensis, Dendranthema morifolium. The concentration of salvianolic acid B was determined by high performance liquid chromatography (HPLC). The method employed a column of Agilent Eclipse XDB-C18 (4.6 mm×250 mm,5 μm) with a mobile phase of 0.5% formic acid (A)-acetonitrile (B) at a temperature 25℃. The gradient elution program was as follow:0~5 min 24% B, 5~6 min 24%~19% B, 6~25 min 19~20% B. The flow rate was 1.0 ml/min, and the injection volume was 10 μl and the detection wavelength was 286 nm. Results The spots in TLC plates were clear and specific. As for salvianolic acid B, the linear range was 20-1280 μg/ml and the equation of linear regression of salvianolic acid B was Y=13.304 X-117.50 (r=0.9999, n=7). The average recovery rate was 96.17% (RSD=1.10%). Conclusion The method was proved to be simple, reliable, reproducible, and could be used in the quality control of Xinnaoling granules.
国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药科技出版社, 2010:附录VI B.
[2]
Koon CM,Woo KS,Leung PC,et al.Salviae Miltiorrhizae Radix and Puerariae Lobatae Radix herbal formula mediates anti-atherosclerosis by modulating key atherogenic events both in vascular smooth muscle cells and endothelial cells[J].J Ethnopharmacol, 2011, 138(1):175-183.
[3]
Ren ZZ, Wang X, Wang SF, et al. Mechanism of action of salvianolic acid B by module-based network analysis[J]. Bio-med Mater Engineer, 2014, 23(1):S1385-S1392.
1. Department of Pharmacy, Changzheng Hospital Affiliated to Second Military Medical University, Shanghai 200433, China;School of Pharmacy, Henan University, Kaifeng 475000, China
2. School of Pharmacy, Henan University, Kaifeng 475000, China
3. Department of Pharmacy, Changzheng Hospital Affiliated to Second Military Medical University, Shanghai 200433, China
Abstract: Objective To establish quality control of Xinnaoling granules. Methods Thin layer chromatography (TLC) methods were used to identify Rhizoma Corydalis, Radix Angelicae dahuricae, Cortex Magnoliae officinalis, Radix Glycyrrhizae, Rhizoma Ligusticum, Ligusticum wallichii, Schisandra Chinensis, Dendranthema morifolium. The concentration of salvianolic acid B was determined by high performance liquid chromatography (HPLC). The method employed a column of Agilent Eclipse XDB-C18 (4.6 mm×250 mm,5 μm) with a mobile phase of 0.5% formic acid (A)-acetonitrile (B) at a temperature 25℃. The gradient elution program was as follow:0~5 min 24% B, 5~6 min 24%~19% B, 6~25 min 19~20% B. The flow rate was 1.0 ml/min, and the injection volume was 10 μl and the detection wavelength was 286 nm. Results The spots in TLC plates were clear and specific. As for salvianolic acid B, the linear range was 20-1280 μg/ml and the equation of linear regression of salvianolic acid B was Y=13.304 X-117.50 (r=0.9999, n=7). The average recovery rate was 96.17% (RSD=1.10%). Conclusion The method was proved to be simple, reliable, reproducible, and could be used in the quality control of Xinnaoling granules.
ZHU Hengmei, LU Guihua, FANG Shuheng, XU Lili. Pain Management with fentanyl transdermal and morphine subcutaneous injection for the patients with advanced liver cancer pain after intervention therapy[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(1): 75-77. doi: 10.3969/j.issn.1006-0111.2017.01.019
Citation:
REN Xinxin, WANG Xianyou, LU Wenquan, Piao Shujuan, GAO Shouhong. Study on quality control of Xinnaoling granules[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 338-342. doi: 10.3969/j.issn.1006-0111.2016.04.014
国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药科技出版社, 2010:附录VI B.
[2]
Koon CM,Woo KS,Leung PC,et al.Salviae Miltiorrhizae Radix and Puerariae Lobatae Radix herbal formula mediates anti-atherosclerosis by modulating key atherogenic events both in vascular smooth muscle cells and endothelial cells[J].J Ethnopharmacol, 2011, 138(1):175-183.
[3]
Ren ZZ, Wang X, Wang SF, et al. Mechanism of action of salvianolic acid B by module-based network analysis[J]. Bio-med Mater Engineer, 2014, 23(1):S1385-S1392.
ZHU Hengmei, LU Guihua, FANG Shuheng, XU Lili. Pain Management with fentanyl transdermal and morphine subcutaneous injection for the patients with advanced liver cancer pain after intervention therapy[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(1): 75-77. doi: 10.3969/j.issn.1006-0111.2017.01.019
ZHU Hengmei, LU Guihua, FANG Shuheng, XU Lili. Pain Management with fentanyl transdermal and morphine subcutaneous injection for the patients with advanced liver cancer pain after intervention therapy[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(1): 75-77. doi: 10.3969/j.issn.1006-0111.2017.01.019
DownLoad: