<sup>19</sup>F核磁共振定量法测定含氟漱口液中氟化钠的含量

华俊杰; 朱海菲; 李育

doi:10.3969/j.issn.1006-0111.2019.06.009

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¹⁹F核磁共振定量法测定含氟漱口液中氟化钠的含量

华俊杰, 朱海菲, 李育

文章导航 > 药学实践与服务 > 2019 > 37(6): 518-520

华俊杰, 朱海菲, 李育. 19F核磁共振定量法测定含氟漱口液中氟化钠的含量[J]. 药学实践与服务, 2019, 37(6): 518-520. doi: 10.3969/j.issn.1006-0111.2019.06.009

引用本文:

华俊杰, 朱海菲, 李育. ¹⁹F核磁共振定量法测定含氟漱口液中氟化钠的含量[J]. 药学实践与服务, 2019, 37(6): 518-520. doi: 10.3969/j.issn.1006-0111.2019.06.009

HUA Junjie, ZHU Haifei, LI Yu. Determination of sodium fluoride in fluoride-containing mouthwash by 19F-NMR[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 518-520. doi: 10.3969/j.issn.1006-0111.2019.06.009

Citation:

HUA Junjie, ZHU Haifei, LI Yu. Determination of sodium fluoride in fluoride-containing mouthwash by ¹⁹F-NMR[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 518-520. doi: 10.3969/j.issn.1006-0111.2019.06.009

¹⁹F核磁共振定量法测定含氟漱口液中氟化钠的含量

doi: 10.3969/j.issn.1006-0111.2019.06.009

海军军医大学药学院上海 200433

Determination of sodium fluoride in fluoride-containing mouthwash by ¹⁹F-NMR

School of Pharmacy, Naval Medical University, Shanghai 200433, China

摘要: 目的建立¹⁹F核磁共振技术测定含氟漱口液中氟化钠含量的方法。方法采用¹⁹F核磁共振技术，以三氟乙酸作为内标物，使用300MHz核磁共振波谱仪，在25℃下采集样品的¹⁹F核磁共振谱，建立内标标准曲线，用于氟化钠定量分析。结果氟化钠在0.1~0.8 mg/ml范围内，线性关系良好，回归方程为A=1.1392C-0.0013，r=0.99995；低、中、高3个浓度的平均回收率分别为99.36%、99.31%、99.65%，RSD分别为0.51%、0.35%、0.54%，含氟漱口液中氟化钠的平均含量为99.30%。结论研究建立的¹⁹F核磁共振方法准确度、精密度较好，能够快速、准确地测定含氟漱口液中氟化钠的含量。
- ¹⁹F-NMR /
- 氟化钠 /
- 含量测定 /
- 定量核磁共振
Abstract: Objective To establish a method for the determination of sodium fluoride in fluoride-containing mouthwash by ¹⁹F-NMR technique. Methods The ¹⁹F-NMR technique was used to establish the relevant standard curve with trifluoroacetic acid as the internal standard.The ¹⁹F-NMR spectrum of the sample was collected at 25℃ using a 300 MHz NMR spectrometer. Results The linear relationship of sodium fluoride was good in the range of 0.1-0.8 mg/ml.The regression equation was A=1.1392C-0.0013,r=0.99995.The average recovery of low,medium and high concentration levels were 99.36%,99.31.% and 99.65%,respectively,and RSD was 0.51%,0.35% and 0.54% respectively.The average content of sodium fluoride in the fluoride mouthwash was 99.30%. Conclusion The method was accurate and precise which could be used to determine the sodium fluoride in fluoride-containing mouthwash.
- ¹⁹F-NMR /
- sodium fluoride /
- determination of content /
- standard curve

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¹⁹F核磁共振定量法测定含氟漱口液中氟化钠的含量

doi: 10.3969/j.issn.1006-0111.2019.06.009

海军军医大学药学院上海 200433

收稿日期: 2019-04-12
修回日期: 2019-07-20

关键词:

¹⁹F-NMR /

氟化钠 /

含量测定 /

定量核磁共振

摘要: 目的建立¹⁹F核磁共振技术测定含氟漱口液中氟化钠含量的方法。方法采用¹⁹F核磁共振技术，以三氟乙酸作为内标物，使用300MHz核磁共振波谱仪，在25℃下采集样品的¹⁹F核磁共振谱，建立内标标准曲线，用于氟化钠定量分析。结果氟化钠在0.1~0.8 mg/ml范围内，线性关系良好，回归方程为A=1.1392C-0.0013，r=0.99995；低、中、高3个浓度的平均回收率分别为99.36%、99.31%、99.65%，RSD分别为0.51%、0.35%、0.54%，含氟漱口液中氟化钠的平均含量为99.30%。结论研究建立的¹⁹F核磁共振方法准确度、精密度较好，能够快速、准确地测定含氟漱口液中氟化钠的含量。