摘要:
目的 探讨微乳薄层色谱法同时分离鉴别心脑脉通胶囊中多种有效成分。 方法 以聚酰胺薄膜为固定相,微乳液为展开剂,对处方中蓝萼香茶菜、葛根、丹参、川芎四味药材进行定性鉴别,并考察了十二烷基硫酸钠(SDS)、油相、水相等因素对分离效果的影响。 结果 以含水量 75% 的微乳液[SDS-正丁醇-正庚烷-水,g/g]-甲酸-丙酮(5:1:1) 为展开剂,可同时分离鉴别心脑脉通中的蓝萼香茶菜、葛根、丹参、川芎,斑点清晰,且阴性对照无干扰,分离效果理想。 结论 微乳薄层色谱法操作简便,结果准确,重现性好,可同时分离鉴别中成药中的多种有效成分。
Abstract:
Objective To investigate the application of microemulsion thin layer chromatography (TLC) for separation and identification of various active components in Xinnaomaitong capsule. Methods The all-grass of Bluecalyx japanese rabodosia, root of Pueraria lobata, root and rhizome of Salvia miltiorrhiza, rhizome of Ligusticum chuanxiong in Xinnaomaitong capsule were separated and identified simultaneously with the polyamide film as stationary phase and the microemulsion as mobile phases. Different factors, such as the sodium dodecyl sulfate (SDS), oil phase and water phase which effect on the separation of the components were evaluated. Results The all-grass of Bluecalyx japanese rabodosia, root of Pueraria lobata, root and rhizome of Salvia miltiorrhiza, rhizome of Ligusticum chuanxiong in Xinnaomaitong capsule were separated and identified simultaneously with mobile phase of microemulsion:formic acid:acetone (5:1:1). The constituent of microemulsion was SDS:butyl alcohol:n-Heptane:water = 6.7:15.8:2.5:75.0 (g/g). The spots were clear, the negative control was no interference, and it showed an ideal separation. Conclusion Microemulsion TLC is simple, accurate, and reproducible. It can identify a variety of active components in traditional Chinese medicine simultaneously.
点击查看大图
下载:
