Analysis of volatile constituents in Caulis Sinomenii by GC-MS
-
摘要: 目的 建立气相色谱-质谱联用(GC-MS)方法分析青风藤中的挥发油成分。 方法 采用水蒸气蒸馏法提取青风藤挥发油,运用GC-MS技术,结合数据库检索对其挥发油进行分离鉴定。对青风藤中的挥发油进行分析。 结果 实验共鉴定出80个化合物,通过与软件中的质谱标准谱图库进行比较,获得匹配的有63个化合物,主要成分为脂肪酸、甾醇、烯烃以及少量的醛类等物质,含量最多的为十八烷酸和十六烷酸。 结论 该实验丰富了青风藤的物质基础研究,为青风藤质量控制提供了更多的参考依据。Abstract: Objective To establish the determination method for chemical constituents of the volatile oil in Sinornenium actum(Thunb.)Rehd.et Wils by gas chromatography-mass spectrometry(GC-MS). Method The volatile oil was extracted from Sinornenium actum(Thunb.)Rehd.et Wils by steam distillation.The constituents of volatile oil were identified by GC-MS technology,which were identified through database index. Results The results showed that after comparing of the gained mass spectrum with the standard, 63 kinds of chemical constituents in all were identified, the major constituents were the fatty acids, sterols, alkenes and a few kind of aldehyde. The most contents were octadecylic acid and hexadecanoic acid, whose relative percentages were 33.78% and 23.23% respectively. Conclusion The experiment improved the basic components study on Caulis Sinomenii, which could provide more reference quality control of Caulis Sinomenii.
-
Key words:
- Sinornenium actum(Thunb.) /
- Rehd.et wils. /
- volatile oil
-
[1] 中国药典2010版.一部[S]. 2010:181. [2] 霍海如,车锡平.青藤碱对离体兔颈上交感神经节的作用[J].中国药理学通报,1988:4(3):168. [3] 张 欣.毛清藤治疗类风湿关节炎1524例临床分析[J].甘肃中医,1996:9(3):22. [4] 王 岩,周莉玲,李 锐.青风藤的研究进展[J].中药材, 2002, 25(3):209. [5] 任 洁,薛兴亚,章飞芳,等.气相色谱-质谱联用分析青风藤挥发油中化学成分[J].世界科学技术-中医药现代化,2006,8(3):21. [6] 江苏新医学院.中药大辞典(上册) [M]. 1997:1234. [7] 瞿发林,董文燊,刘 蓉,杨 婷,等.不同产地青风藤中青藤碱的含量比较[J].药物研究,2010,19(18):32. -
点击查看大图
计量
- 文章访问数: 5130
- HTML全文浏览量: 338
- PDF下载量: 378
- 被引次数: 0
下载:
