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YAN Bing, DING An-wei, ZHANG Li. Protective effects of aqueous extract of Erzhi pill on hepatocytes injured by CCl4 or H2O2[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(6): 433-439,436.
Citation:
YAN Bing, DING An-wei, ZHANG Li. Protective effects of aqueous extract of Erzhi pill on hepatocytes injured by CCl4 or H2O2[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(6): 433-439,436.
Protective effects of aqueous extract of Erzhi pill on hepatocytes injured by CCl4 or H2O2
Jiangsu Key Laboratory for TCM Formulae Research,Nanjing University of Chinese Medicine,Nanjing 210046,China;Dept.of Pharmaceuticals,Nanjing General Hospital of Nanjing Military Region,Nanjing 210002,China
2.
Jiangsu Key Laboratory for TCM Formulae Research,Nanjing University of Chinese Medicine,Nanjing 210046,China
Received Date: 2010-04-30
Rev Recd Date:
2010-05-27
Abstract
Objective To study the protective effects and mechanism of aqueous extract of Erzhi pill on hepatocytes injured by CCL4 or H2O2. Methods The L-O2 hepatocytes were incubated and then injured by CCl4.The levels of AST,ALT,MDA and SOD in cultural supernatant were detected by general Methods.Cell viability was assayed by MTT method. Results The elevation of MDA content of hepatocytes and AST,ALT level in supernatant of hepatocytes,and the loss of SOD activit y induced by CCl4 were restored remarkably by AEEP(0.32～40 μg/ml).The elevation of ALT,AST level in the supernatant of hepatocytes and MDA content of hepatocytes,and the loss of SOD activity induced by H2O2 were improved significantly by AEEP(0.32～40 μg/ml)treatment. Conclusion The Results suggest that AEEP possess direct protective action on hepatocytes in vitro injured by CCl4 or H2O2.These might be associated with its anti-oxidative activity.
1. Jiangsu Key Laboratory for TCM Formulae Research,Nanjing University of Chinese Medicine,Nanjing 210046,China;Dept.of Pharmaceuticals,Nanjing General Hospital of Nanjing Military Region,Nanjing 210002,China
2. Jiangsu Key Laboratory for TCM Formulae Research,Nanjing University of Chinese Medicine,Nanjing 210046,China
Abstract: Objective To study the protective effects and mechanism of aqueous extract of Erzhi pill on hepatocytes injured by CCL4 or H2O2. Methods The L-O2 hepatocytes were incubated and then injured by CCl4.The levels of AST,ALT,MDA and SOD in cultural supernatant were detected by general Methods.Cell viability was assayed by MTT method. Results The elevation of MDA content of hepatocytes and AST,ALT level in supernatant of hepatocytes,and the loss of SOD activit y induced by CCl4 were restored remarkably by AEEP(0.32～40 μg/ml).The elevation of ALT,AST level in the supernatant of hepatocytes and MDA content of hepatocytes,and the loss of SOD activity induced by H2O2 were improved significantly by AEEP(0.32～40 μg/ml)treatment. Conclusion The Results suggest that AEEP possess direct protective action on hepatocytes in vitro injured by CCl4 or H2O2.These might be associated with its anti-oxidative activity.
YAN Bing, DING An-wei, ZHANG Li. Protective effects of aqueous extract of Erzhi pill on hepatocytes injured by CCl4 or H2O2[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(6): 433-439,436.
Citation:
YAN Bing, DING An-wei, ZHANG Li. Protective effects of aqueous extract of Erzhi pill on hepatocytes injured by CCl4 or H2O2[J]. Journal of Pharmaceutical Practice and Service, 2010, 28(6): 433-439,436.
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